Non-anaphylactic surface-exposed peptides of the major birch pollen allergen, Bet v 1, for preventive vaccination
| Autor: | Birgit Linhart, Arnulf Josef Hartl, Wolfgang R. Sperr, Rudolf Valenta, M. Focke, Ursula Wiedermann, Dietrich Kraft, Josef Thalhamer, Peter Valent |
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| Rok vydání: | 2004 |
| Předmět: |
Hypersensitivity
Immediate Allergy medicine.medical_treatment Immunology Enzyme-Linked Immunosorbent Assay medicine.disease_cause Basophil degranulation Histamine Release Epitope Microbiology Allergic sensitization Epitopes Mice Allergen Blocking antibody otorhinolaryngologic diseases medicine Animals Humans Immunology and Allergy Amino Acid Sequence Betula Skin Tests Desensitization (medicine) B-Lymphocytes Vaccines business.industry Allergens Antigens Plant Immunoglobulin E medicine.disease Basophils Protein Structure Tertiary Rats Desensitization Immunologic Immunoglobulin G Pollen Rabbits Peptides business Anaphylaxis |
| Zdroj: | Clinical Experimental Allergy. 34:1525-1533 |
| ISSN: | 1365-2222 0954-7894 |
| Popis: | Summary Background Almost 100 million allergic patients are sensitized to the major birch pollen allergen, Bet v 1, a 17 kDa protein containing most of the IgE epitopes present in pollens of trees belonging to the Fagales order and plant-derived food. Objective Our aim was to develop an approach for the rational design of B cell epitope-derived, non-allergenic peptide allergy vaccines. Methods According to the three-dimensional (3-D) structure of birch pollen allergen, Bet v 1, six peptides comprising 25–32 preferably solvent-exposed amino acids were synthesized. Results Because of lack of secondary structure, the peptides showed no allergenic activity in allergic patients. In a mouse model of birch pollen allergy, peptide vaccination induced Bet v 1-specific IgG and prevented IgE-mediated allergic sensitization to Bet v 1. The protective role of peptide-induced blocking antibodies is demonstrated by inhibition of allergic patients IgE binding to the allergen and by blocking of allergen-induced basophil degranulation. Conclusion Our results indicate the mechanistic importance of blocking antibodies for allergy vaccination and present a B cell epitope-based approach for the rational design of safe peptide allergy vaccines whenever the structure of the disease-eliciting allergen is known. |
| Databáze: | OpenAIRE |
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