Gut microbiota of children with atopic dermatitis: Controlled study in the metropolitan region of São Paulo, Brazil
Autor: | Mirian Silva Carmo-Rodrigues, Humberto Bezerra de Araujo-Filho, Lígia C. F. L. Melli, Carolina Santos Mello, Dirceu Solé, Mauro Batista de Morais, Soraia Tahan, Antonio Carlos Campos Pignatari |
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Rok vydání: | 2020 |
Předmět: |
Male
Pulmonary and Respiratory Medicine Salmonella Allergy Firmicutes Immunology Gut flora medicine.disease_cause Dermatitis Atopic 03 medical and health sciences 0302 clinical medicine Lactobacillus medicine Humans Immunology and Allergy Child Bifidobacterium biology business.industry Bacteroidetes General Medicine Atopic dermatitis biology.organism_classification medicine.disease Gastrointestinal Microbiome Cross-Sectional Studies 030228 respiratory system Child Preschool Female business Brazil 030215 immunology |
Zdroj: | Allergologia et Immunopathologia. 48:107-115 |
ISSN: | 0301-0546 |
Popis: | Background It is possible that imbalances in the composition of the gut microbiota or the relationship of the microbiota with the host may be implicated in the origin of allergy. Therefore, we studied the intestinal microbiota of children with atopic dermatitis (AD). Methods Cross-sectional study with 81 children aged 5–11; 23 with AD and 58 controls. Surveys were conducted to obtain demographic, socioeconomic and neonatal data. Diagnosis of AD was made based on the International Study of Asthma and Allergies in Childhood (ISAAC) questionnaire. Eubacteria, Bacteroidetes, Firmicutes, B. fragilis, E. coli, Lactobacillus spp., S. aureus, E. faecalis, Salmonella spp., M. smithii, Bifidobacterium spp., C. difficile and C. perfringens were quantified using real-time PCR. Results The analysis showed an association between presence of C. difficile (OR: 5.88; 95 % CI: 1.24; 27.98), greater abundance of bifidobacteria (OR: 11.09; 95 % CI: 2.14; 57.39) and a lower abundance of lactobacilli (OR: 0.07; 95 % CI: 0.01; 0.51) in the gut microbiota of children with AD. Counts of Eubacteria (0,05 × 103 and 8.49 × 103), B. fragilis (0.72 × 109 and 4.5 × 109), Lactobacillus spp. (0.02 × 108 and 0.38 × 108), E. coli (0.13 × 109 and 1.52 × 109) and M. smithii (0.02 × 108 and 0.31 × 108) were lower in children with AD (P Conclusions This study confirmed that children living in the metropolitan area of Sao Paulo (Brazil) with AD have a different microbiota pattern with higher prevalence of C. difficile, lower abundance of Lactobacillus and greater abundance of bifidobacteria, regardless of socioeconomic status. |
Databáze: | OpenAIRE |
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