Antigen CD34+ marrow cells engraft lethally irradiated baboons
| Autor: | Glenn H. Knitter, William I. Bensinger, C. D. Buckner, Robert G. Andrews, Irwin D. Bernstein, Berenson Rj, Kalamasz Df |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
medicine.medical_treatment
Population CD34 Cell Separation Hematopoietic stem cell transplantation Biology Postoperative Complications Antigen Bone Marrow medicine Animals Progenitor cell education Bone Marrow Transplantation education.field_of_study Graft Survival Hematopoietic Stem Cell Transplantation Syndrome General Medicine Hematopoietic Stem Cells Antigens Differentiation Molecular biology Haematopoiesis Phenotype medicine.anatomical_structure Radiation Chimera Immunology Bone marrow Stem cell Papio Research Article |
| Zdroj: | Journal of Clinical Investigation. 81:951-955 |
| ISSN: | 0021-9738 |
| DOI: | 10.1172/jci113409 |
| Popis: | The CD34 antigen is present on 1-4% of human marrow cells including virtually all hematopoietic progenitors detected by in vitro assays. Since the anti-CD34 monoclonal antibody 12-8 reacts with a similar marrow population in baboons, it was possible to test whether this antigen is expressed by stem cells responsible for hematopoietic reconstitution in vivo. CD34+ cells were enriched from marrows of five baboons using avidin-biotin immunoadsorption. After lethal irradiation, the five animals were given 15-27 X 10(6) autologous marrow cells (3.2-4.4 X 10(6) cells/kg) containing 65-91% CD34+ cells. All animals achieved granulocyte counts greater than 1,000/mm3 and platelet counts greater than 20 X 10(3)/mm3 by 13-24 d posttransplant and subsequently developed normal peripheral blood counts. Two additional animals received 184 and 285 X 10(6) marrow cells/kg depleted of CD34+ cells. One animal died at day 29 without engraftment, while the other had pancytopenia for greater than 100 d posttransplant. The data suggest that stem cells responsible for hematopoietic reconstitution are CD34+. |
| Databáze: | OpenAIRE |
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