T-DNA integration into Arabidopsis genome depends on sequences of pre-insertion sites
| Autor: | Loïc Lepiniec, Richard DeRose, Véronique Brunaud, Franck Samson, Alain Lecharny, Corinne Cruaud, Bertrand Dubreucq, Sandrine Balzergue, Michel Caboche, Sébastien Aubourg, Georges Pelletier, Nicole Bechtold, Stéphanie Chauvin |
|---|---|
| Přispěvatelé: | Unité de recherche en génomique végétale (URGV), Institut National de la Recherche Agronomique (INRA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Biologie des Semences (LBS), Institut National de la Recherche Agronomique (INRA)-Institut National Agronomique Paris-Grignon (INA P-G), Unité de recherche Génétique et amélioration des plantes (GAP), Institut National de la Recherche Agronomique (INRA), Centre National de la Recherche Scientifique (CNRS)-Université d'Évry-Val-d'Essonne (UEVE)-Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
0106 biological sciences
Base pair 5' Flanking Region [SDV]Life Sciences [q-bio] 5' flanking region Scientific Report Arabidopsis Sequence Homology Biology 01 natural sciences Biochemistry 3' Flanking Region 03 medical and health sciences chemistry.chemical_compound D-loop Genetics 5' REGION FLANQUANTE DNA Integration Molecular Biology 3' REGION FLANQUANTE 030304 developmental biology 0303 health sciences Gene Transfer Techniques DNA Plants Genetically Modified chemistry T-DNA Human genome In vitro recombination Genome Plant 010606 plant biology & botany |
| Zdroj: | EMBO Reports EMBO Reports, 2002, 3 (12), pp.1152-1157. ⟨10.1093/embo-reports/kvf237⟩ EMBO Reports, EMBO Press, 2002, 3 (12), pp.1152-1157. ⟨10.1093/embo-reports/kvf237⟩ |
| ISSN: | 1469-221X 1469-3178 |
| DOI: | 10.1093/embo-reports/kvf237⟩ |
| Popis: | A statistical analysis of 9000 flanking sequence tags characterizing transferred DNA (T-DNA) transformants in Arabidopsis sheds new light on T-DNA insertion by illegitimate recombination. T-DNA integration is favoured in plant DNA regions with an A-T-rich content. The formation of a short DNA duplex between the host DNA and the left end of the T-DNA sets the frame for the recombination. The sequence immediately downstream of the plant A-T-rich region is the master element for setting up the DNA duplex, and deletions into the left end of the integrated T-DNA depend on the location of a complementary sequence on the T-DNA. Recombination at the right end of the T-DNA with the host DNA involves another DNA duplex, 2–3 base pairs long, that preferentially includes a G close to the right end of the T-DNA. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text