Angiotensin II-induced superoxide and decreased glutathione in proximal tubules: effect of dietary fructose
| Autor: | Jeffrey L. Garvin, Nianxin Yang, Agustin Gonzalez-Vicente |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Dietary Sugars Pyridones Physiology Fructose 030204 cardiovascular system & hematology medicine.disease_cause Antioxidants Kidney Tubules Proximal Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Superoxides Internal medicine medicine Animals Kidney NADPH oxidase biology Superoxide Angiotensin II Acetophenones NADPH Oxidases Nephrons Glutathione Rats Oxidative Stress 030104 developmental biology medicine.anatomical_structure Endocrinology chemistry biology.protein Pyrazoles Oxidative stress Research Article |
| Zdroj: | Am J Physiol Renal Physiol |
| ISSN: | 1522-1466 1931-857X |
| DOI: | 10.1152/ajprenal.00462.2019 |
| Popis: | Angiotensin II exacerbates oxidative stress in part by increasing superoxide ([Formula: see text]) production by many renal tissues. However, whether it does so in proximal tubules and the source of [Formula: see text] in this segment are unknown. Dietary fructose enhances the stimulatory effect of angiotensin II on proximal tubule Na+reabsorption, but whether this is true for oxidative stress is unknown. We hypothesized that angiotensin II causes proximal nephron oxidative stress in part by stimulating NADPH oxidase (NOX)4-dependent [Formula: see text] production and decreasing the amount of the antioxidant glutathione, and this is exacerbated by dietary fructose. We measured basal and angiotensin II-stimulated [Formula: see text] production with and without inhibitors, NOX1 and NOX4 expression, and total and reduced glutathione (GSH) in proximal tubules from rats drinking either tap water (control) or 20% fructose. Angiotensin II (10 nM) increased [Formula: see text] production by 113 ± 42 relative light units·mg protein−1·s−1in controls and 401 ± 74 relative light units·mg protein−1·s−1with 20% fructose ( n = 11 for each group, P < 0.05 vs. control). Apocynin and the Nox1/4 inhibitor GKT136901 prevented angiotensin II-induced increases in both groups. NOX4 expression was not different between groups. NOX1 expression was undetectable. Angiotensin II decreased GSH by 1.8 ± 0.8 nmol/mg protein in controls and by 4.2 ± 0.9 nmol/mg protein with 20% fructose ( n = 18 for each group, P < 0.047 vs. control). We conclude that 1) angiotensin II causes oxidative stress in proximal tubules by increasing [Formula: see text] production by NOX4 and decreasing GSH and 2) dietary fructose enhances the ability of angiotensin II to stimulate [Formula: see text] and diminish GSH, thereby exacerbating oxidative stress in this segment. |
| Databáze: | OpenAIRE |
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