Effect of a resin-modified calcium silicate cement on inflammatory cell infiltration and reparative dentin formation after pulpotomy in rat molars
| Autor: | Shoji Takenaka, Nagako Yoshiba, Razi Saifullah Ibn Belal, Yuichiro Noiri, Naoto Ohkura, Naoki Edanami, Kunihiko Yoshiba |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Molar
Pulpotomy Dentistry Odontoblast differentiation Dentin Secondary chemistry.chemical_compound stomatognathic system medicine Animals Aluminum Compounds General Dentistry Dental Pulp Cement Chemistry business.industry Silicates Oxides X-Ray Microtomography Calcium Compounds medicine.disease Rats Resin Cements Drug Combinations Calcium silicate Pulp (tooth) business Infiltration (medical) Immunostaining |
| Zdroj: | Australian endodontic journal : the journal of the Australian Society of Endodontology IncReferences. 48(2) |
| ISSN: | 1747-4477 |
| Popis: | Resin monomers and polymerisation initiators have been shown to be cytotoxic for pulp cells and to disturb odontoblast differentiation. This study aimed to compare the effect of a resin-modified calcium silicate cement (TheraCal LC; TC) and a resin-free calcium silicate cement (ProRoot MTA; PR) on pulpal healing after pulpotomy. Pulpotomy was performed on the maxillary first molars of 8-week-old rats using either PR or TC. After 1, 3, 7, 14 and 28 days, pulpal responses were assessed by micro-computed tomography, haematoxylin-eosin staining and immunostaining against CD68, which is a pan-macrophage marker. The results showed that pulpotomy with TC induced persistent infiltration of inflammatory cells, including CD68-positive macrophages, and delayed the formation of reparative dentin as compared with that with PR, although both materials allowed pulpal healing over the long term. Therefore, resin-modified TC was not as biocompatible nor bioinductive as resin-free PR when applied on the healthy pulp of rat molars. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text