Conducting polymer-based impedimetric aptamer biosensor for in situ detection
| Autor: | Wei Liao, Xinyan Tracy Cui, Nicolas Alexander Alba, Brad A. Randall |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
In situ
Polymers Aptamer Nanotechnology Biosensing Techniques Polypyrrole Sensitivity and Specificity Biochemistry Analytical Chemistry chemistry.chemical_compound Electric Impedance Electrochemistry Pyrroles In Situ Hybridization Platelet-Derived Growth Factor Conductive polymer chemistry.chemical_classification Chromatography Base Sequence Reproducibility of Results Substrate (chemistry) Polymer Aptamers Nucleotide Immunoglobulin E Dielectric spectroscopy chemistry Microscopy Electron Scanning Microelectrodes Biosensor |
| Zdroj: | Analytical and Bioanalytical Chemistry. 392:861-864 |
| ISSN: | 1618-2650 1618-2642 |
| DOI: | 10.1007/s00216-008-2354-8 |
| Popis: | We have successfully developed the first prototype of an electrochemical protein biosensor using polypyrrole as the substrate and doped aptamer as the probe. The sensitivity for a specific target is 10 ng/ml. Two targets, platelet-derived growth factor and immunoglobulin E, have been tested. In both cases the sensor exhibited high specificity and the signal was found to increase as the target concentration increased. |
| Databáze: | OpenAIRE |
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