Intravenous administration of synthetic platelets (SynthoPlate) in a mouse liver injury model of uncontrolled hemorrhage improves hemostasis
| Autor: | DaShawn A. Hickman, Norman F. Luc, Matthew D. Neal, Mitchell Dyer, Christa L. Pawlowski, Shannon Haldeman, Anirban Sen Gupta, Patricia Loughran |
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| Rok vydání: | 2018 |
| Předmět: |
Blood Platelets
Male 0301 basic medicine Platelet Transfusion Shock Hemorrhagic 030204 cardiovascular system & hematology Critical Care and Intensive Care Medicine Article Traumatic Hemorrhage Mice 03 medical and health sciences 0302 clinical medicine Blood Substitutes Animals Medicine Platelet Infusions Intravenous Liver injury Hemostasis business.industry Extramural medicine.disease Mice Inbred C57BL Disease Models Animal 030104 developmental biology Platelet transfusion Liver Anesthesia Shock (circulatory) Surgery medicine.symptom business |
| Zdroj: | Journal of Trauma and Acute Care Surgery. 84:917-923 |
| ISSN: | 2163-0763 2163-0755 |
| Popis: | BACKGROUND: Clinical resuscitative treatment of traumatic hemorrhage involves transfusion of RBC, platelets and plasma in controlled ratios. However, use of such blood components, especially platelets, present many challenges including availability, portability, contamination risks, and short shelf-life, which limit the use of platelet transfusions outside of large trauma centers such as remote civilian hospitals and austere pre-hospital settings. This has prompted significant research in platelet substitutes that may resolve the above issues while providing platelet-mimetic hemostatic action. In this framework, we have developed a synthetic platelet surrogate, SynthoPlate(™), by integrative decoration of platelet function mimetic peptides on a biocompatible lipid nanovesicle platform. We have previously demonstrated hemostatic capability of SynthoPlate(™) in correcting tail-bleeding time in thrombocytopenic mice. Building on this, we hypothesized that SynthoPlate(™) transfusion would decrease bleeding in a murine model of acute hemorrhagic shock. METHODS: A validated model of uncontrolled intraperitoneal hemorrhage, via liver laceration was utilized to induce hemorrhagic shock in mice. SynthoPlate(™), control (unmodified) particles, and normal saline were administered as pre-treatment and recue infusions to mice undergoing liver laceration and evaluated for hemostatic benefit by determining differences in blood loss and monitoring real-time hemodynamic data. RESULTS: Pre-treatment SynthoPlate(™) transfusion resulted in significant reduction of blood loss following hemorrhage, compared to control particles or normal saline treatment (0.86±0.16g CP vs. 0.84±0.13g NS vs. 0.68±0.09g SynthoPlate(™), p |
| Databáze: | OpenAIRE |
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