Application of response surface methodology and artificial neural networks for optimization of recombinant Oryza sativa non-symbiotic hemoglobin 1 production by Escherichia coli in medium containing byproduct glycerol

Autor: Hugo D. Martínez, Alberto A. Iglesias, Pablo C. Giordano, Héctor C. Goicoechea, Alejandro J. Beccaria
Rok vydání: 2009
Předmět:
Glycerol
Environmental Engineering
Sodium
chemistry.chemical_element
Bioengineering
INGENIERÍAS Y TECNOLOGÍAS
Biology
Biotecnología Industrial
Hemoglobins
chemistry.chemical_compound
Escherichia coli
By-product
Yeast extract
Biomass
Response surface methodology
Waste Management and Disposal
Plant Proteins
Recombination
Genetic
Chromatography
Renewable Energy
Sustainability and the Environment
ARTIFICIAL NEURAL NETWORKS
Ciencias Químicas
Reproducibility of Results
Oryza
General Medicine
Bioprocesamiento Tecnológico
Biocatálisis
Fermentación
Culture Media
chemistry
Biochemistry
Tryptone
Yield (chemistry)
Biodiesel production
BYPRODUCT GLYCEROL
Química Analítica
Neural Networks
Computer
ESCHERICHIA COLI
RECOMBINANT PROTEIN
RESPONSE SURFACE METHODOLOGY
CIENCIAS NATURALES Y EXACTAS
Biotechnology
Zdroj: Bioresource technology. 101(19)
ISSN: 1873-2976
Popis: Production of recombinant Oryza sativa non-symbiotic hemoglobin 1 (OsHb1) by Escherichia coli was maximized in shake-flask cultures in media containing tryptone, yeast extract, sodium chloride and byproduct glycerol from biodiesel production. Response surface methodology (RSM) and artificial neural networks (ANNs), followed by multiple response optimization through a desirability function were applied to evaluate the amount of OsHb1 produced. The results obtained by the application of ANNs were more reliable since better statistical parameters were obtained. The optimal conditions were (g L-1), tryptone, 42.69; yeast extract, 20.11; sodium chloride, 17.77; and byproduct glycerol, 0.33. A maximum recombinant protein concentration of 3.50 g L-1 and a minimum biomass concentration of 18.48 g L -1 were obtained under these conditions. Although the concentrations of tryptone, yeast extract and sodium chloride are relatively high, the increase in the yield with respect to biomass formed (YP/X) overcomes this disadvantage. Fil: Giordano, Pablo César. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina Fil: Martínez, Hugo D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina Fil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina Fil: Beccaria, Alejandro José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Databáze: OpenAIRE
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