Collagen fibrils and proteoglycans of peripheral and central stroma of the keratoconus cornea - Ultrastructure and 3D transmission electron tomography

Autor:	Adnan A. Khan, Robert Barsotti, Turki M Almubrad, Saeed Akhtar, Aljoharah Alkanaan, Omar Kirat
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	Adult 0301 basic medicine Electron Microscope Tomography Pathology medicine.medical_specialty Keratoconus Corneal Stroma Cells lcsh:Medicine Article Collagen fibril Cornea Fibril-Associated Collagens 03 medical and health sciences 0302 clinical medicine Microscopy Electron Transmission Stroma medicine Humans lcsh:Science Multidisciplinary Chemistry lcsh:R Translational research medicine.disease eye diseases Extracellular Matrix Peripheral Corneal Disorder 030104 developmental biology medicine.anatomical_structure Electron tomography Microfibrils 030221 ophthalmology & optometry Ultrastructure Proteoglycans lcsh:Q Collagen sense organs
Zdroj:	Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019) Scientific Reports
ISSN:	2045-2322
Popis:	Keratoconus (KC) is a progressive corneal disorder in which vision gradually deteriorates as a result of continuous conical protrusion and the consequent altered corneal curvature. While the majority of the literature focus on assessing the center of this diseased cornea, there is growing evidence of peripheral involvement in the disease process. Thus, we investigated the organization of collagen fibrils (CFs) and proteoglycans (PGs) in the periphery and center of KC corneal stroma. Three-dimensional transmission electron tomography on four KC corneas showed the degeneration of microfibrils within the CFs and disturbance in the attachment of the PGs. Within the KC corneas, the mean CF diameter of the central-anterior stroma was significantly (p ˂ 0.001) larger than the peripheral-anterior stroma. The interfibrillar distance of CF was significantly (p ˂ 0.001) smaller in the central stroma than in the peripheral stroma. PGs area and the density in the central KC stroma were larger than those in the peripheral stroma. Results of the current study revealed that in the pre- Descemet’s membrane stroma of the periphery, the degenerated CFs and PGs constitute biomechanically weak lamellae which are prone to disorganization and this suggests that the peripheral stroma plays an important role in the pathogenicity of the KC cornea.
Databáze:	OpenAIRE
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