Identification and characterisation of small-molecule inhibitors of Rv3097c-encoded lipase (LipY) of Mycobacterium tuberculosis that selectively inhibit growth of bacilli in hypoxia
| Autor: | S.P. Vishnoi, Brahm S. Srivastava, Supriya Singh, Kuldeep K. Roy, Vivek Kr. Kashyap, Anil K. Saxena, Anil Kumar, Ranjana Srivastava, Laurent Kremer |
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| Rok vydání: | 2013 |
| Předmět: |
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Molecular Microbiology (medical) Bacilli Cell Survival Protein Conformation Microbiology law.invention Mycobacterium tuberculosis chemistry.chemical_compound law Chlorocebus aethiops medicine Animals Pharmacology (medical) Anaerobiosis Enzyme Inhibitors Lipase Vero Cells chemistry.chemical_classification Microbial Viability Molecular Structure biology Latent tuberculosis General Medicine medicine.disease biology.organism_classification Anti-Bacterial Agents Infectious Diseases Enzyme chemistry Biochemistry biology.protein Recombinant DNA Growth inhibition Mycobacterium |
| Zdroj: | International Journal of Antimicrobial Agents. 42:27-35 |
| ISSN: | 0924-8579 |
| DOI: | 10.1016/j.ijantimicag.2013.03.007 |
| Popis: | The mycobacterial Rv3097c-encoded lipase LipY is considered as a true lipase involved in the hydrolysis of triacylglycerol stored in lipid inclusion bodies for the survival of dormant mycobacteria. To date, orlistat is the only known LipY inhibitor. In view of the important emerging role of this enzyme, a search for small-molecule inhibitors of LipY was made, leading to the identification of some new compounds ( 8a–8d , 8f , 8h and 8i ) with potent inhibitory activities against recombinant LipY, with no cytotoxicity [50% inhibitory concentration (CC 50 ) ≥500 μg/mL]. The compounds 6a , 8c and 8f potently inhibited (>90%) the growth of Mycobacterium tuberculosis H 37 Rv grown under hypoxia (oxygen-depleted condition) but had no effect on aerobically grown bacilli, suggesting that these new small molecules are highly selective towards the growth inhibition of hypoxic cultures of M. tuberculosis and hence provide new leads for combating latent tuberculosis. |
| Databáze: | OpenAIRE |
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