Biomimetic microenvironmental preconditioning enhance neuroprotective properties of human mesenchymal stem cells derived from Wharton's Jelly (WJ-MSCs)
| Autor: | Krystyna Domanska-Janik, Zuzanna Kuczynska, Anna Figiel-Dabrowska, Marzena Zychowicz, Anna Sarnowska, Filip A Dabrowski, Leonora Buzanska, Wioletta Lech |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Population
lcsh:Medicine Stem cells Neuroprotection Hippocampus Article Umbilical Cord Biomaterials Biomimetics Wharton's jelly Animals Humans Wharton Jelly Rats Wistar Stem Cell Niche lcsh:Science education Regeneration and repair in the nervous system Cells Cultured Cell Proliferation education.field_of_study Multidisciplinary Chemistry lcsh:R Mesenchymal stem cell Cell Differentiation Hydrogels Mesenchymal Stem Cells Antigens Differentiation In vitro Coculture Techniques Cell biology Rats Cytokines lcsh:Q Platelet lysate Stem cell Ex vivo Stem-cell niche |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-15 (2020) |
| ISSN: | 2045-2322 |
| Popis: | Tuning stem cells microenvironment in vitro may influence their regenerative properties. In this study Wharton's Jelly-derived mesenchymal stem cells (WJ-MSCs) were encapsulated in 3D hydrogels derived from human fibrin (FB) or platelet lysate (PL) and the oxygen level was adjusted to physiological normoxia (5% O2). The influence of the type of the scaffold and physiological normoxia conditions was tested on the WJ-MSCs' survivability, proliferation, migratory potential, the level of expression of selected trophic factors, cytokines, and neural markers. Encapsulated WJ-MSCs revealed high survivability, stable proliferation rate, and ability to migrate out of the hydrogel and the up-regulated expression of all tested factors, as well as the increased expression of neural differentiation markers. Physiological normoxia stimulated proliferation of encapsulated WJ-MSCs and significantly enhanced their neuronal, but not glial, differentiation. Ex vivo studies with indirect co-culture of organotypic hippocampal slices and cell-hydrogel bio-constructs revealed strong neuroprotective effect of WJ-MSCs against neuronal death in the CA1 region of the rat hippocampus. This effect was potentiated further by FB scaffolds under 5% O2 conditions. Our results indicating significant effect of oxygen and 3D cytoarchitecture suggest the urgent need for further optimization of the microenvironmental conditions to improve therapeutical competence of the WJ-MSCs population. |
| Databáze: | OpenAIRE |
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