Probing Biosensing Interfaces With Single Molecule Localization Microscopy (SMLM)
Autor: | Wei Yin, Xiaoyu Cheng |
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Rok vydání: | 2021 |
Předmět: |
Single molecule localization
0303 health sciences Fluorescence-lifetime imaging microscopy Materials science Mini Review Cellular imaging Resolution (electron density) Nanotechnology 02 engineering and technology General Chemistry biosensors 021001 nanoscience & nanotechnology interfaces Chemistry 03 medical and health sciences Molecular level fluorescence imaging Microscopy single molecules 0210 nano-technology QD1-999 Biosensor super-resolution imaging 030304 developmental biology |
Zdroj: | Frontiers in Chemistry Frontiers in Chemistry, Vol 9 (2021) |
ISSN: | 2296-2646 |
DOI: | 10.3389/fchem.2021.655324 |
Popis: | Far field single molecule localization microscopy (SMLM) has been established as a powerful tool to study biological structures with resolution far below the diffraction limit of conventional light microscopy. In recent years, the applications of SMLM have reached beyond traditional cellular imaging. Nanostructured interfaces are enriched with information that determines their function, playing key roles in applications such as chemical catalysis and biological sensing. SMLM enables detailed study of interfaces at an individual molecular level, allowing measurements of reaction kinetics, and detection of rare events not accessible to ensemble measurements. This paper provides an update to the progress made to the use of SMLM in characterizing nanostructured biointerfaces, focusing on practical aspects, recent advances, and emerging opportunities from an analytical chemistry perspective. |
Databáze: | OpenAIRE |
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