Cross-sectional study of the relationships among bedding materials, bedding bacteria counts, and intramammary infection in late-lactation dairy cows
| Autor: | E. Royster, J. Timmerman, Sandra Godden, Brian A Crooker, S.M. Rowe, Michael P. Boyle |
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| Rok vydání: | 2019 |
| Předmět: |
Klebsiella
Bedding Staphylococcus 03 medical and health sciences Animal science Mammary Glands Animal Risk Factors Streptococcal Infections Bedding Material Genetics medicine Prevalence Animals Lactation Mastitis Bovine 030304 developmental biology 0303 health sciences biology Incidence (epidemiology) 0402 animal and dairy science Bedding and Linens Streptococcus 04 agricultural and veterinary sciences Staphylococcal Infections biology.organism_classification medicine.disease 040201 dairy & animal science Manure Bacterial Load Mastitis Coliform bacteria Klebsiella Infections Cross-Sectional Studies Logistic Models Milk Herd Animal Science and Zoology Cattle Female Food Science |
| Zdroj: | Journal of dairy science. 102(12) |
| ISSN: | 1525-3198 |
| Popis: | Objectives of this study were to (1) describe the intramammary infection (IMI) prevalence and pathogen profiles in quarters of cows approaching dry-off in US dairy herds, (2) compare IMI prevalence in quarters of cows exposed to different bedding material types, and (3) identify associations between bedding bacteria count and IMI in cows approaching dry-off. Eighty herds using 1 of 4 common bedding materials (manure solids, organic non-manure, new sand, and recycled sand) were recruited in a multi-site cross-sectional study. Each herd was visited twice for sampling. At each visit, aseptic quarter-milk samples were collected from 20 cows approaching dry-off (>180 d pregnant). Samples of unused and used bedding were also collected. Aerobic culture was used to determine the IMI status of 10,448 quarters and to enumerate counts (log10 cfu/mL) of all bacteria, Staphylococcus spp., Streptococcus spp. and Streptococcus-like organisms (SSLO), coliforms, Klebsiella spp., noncoliform gram-negatives, Bacillus spp., and Prototheca spp. in unused (n = 148) and used (n = 150) bedding. The association between bedding bacteria count and IMI was determined using multivariable logistic regression with mixed effects. Quarter-level prevalence of IMI was 21.1%, which was primarily caused by non-aureus Staphylococcus spp. (11.4%) and SSLO (5.6%). Only modest differences in IMI prevalence were observed between the 4 common bedding material types. Counts of all bacteria in unused bedding was positively associated with odds of IMI caused by any pathogen [ALL-IMI; odds ratio (OR) = 1.08]. A positive association was also observed for counts of SSLO in unused bedding and SSLO-IMI (OR = 1.09). These patterns of association were generally consistent across the 4 common bedding materials. In contrast, the association between counts of all bacteria in used bedding and ALL-IMI varied by bedding type, with positive associations observed in quarters exposed to manure solids (OR = 2.29) and organic non-manure (OR = 1.51) and a negative association in quarters exposed to new sand (OR = 0.47). Findings from this study suggest that quarter-level IMI prevalence in late-lactation cows is low in US dairy herds. Furthermore, bedding material type may not be an important risk factor for IMI in late lactation. Higher levels of bacteria in bedding may increase IMI prevalence at dry-off in general, but this relationship is likely to vary according to bedding material type. |
| Databáze: | OpenAIRE |
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