What is the best wavelength for the measurement of hemolysis index?

Autor: Yutaka Suehiro, Akiyo Ishiguro, Naoko Okayama, Masamune Aihara, Takahiro Yamasaki, Mitsuaki Nishioka, Hidekazu Mizuno, Akihiro Morishige, Yuki Morikawa, Reo Kawano, Toshihiko Kobayashi, Tomihisa Kogo, Aki Fujinaga, Fumiya Takagi
Rok vydání: 2020
Předmět:
Zdroj: Clinica chimica acta; international journal of clinical chemistry. 510
ISSN: 1873-3492
Popis: Background Hemolysis is a common problem in the handling of serum specimens. The hemolysis index (HI) provides a warning of hemolysis in auto-analyzers. However, HI has not been standardized, and each laboratory’s original method is applied. Especially, the wavelength used for HI measurement is different in each laboratory. Thus, we investigated the warning ability of HI at various wavelengths. Methods We selected 4 wavelength types, and each HI was measured and calculated (410 nm/HI-1, 451 nm/HI-2, 545 nm/HI-3, and 571 nm/HI-4). To compare the 4 HI types, we investigated the influence of 3 interference components using artificially hemolyzed specimens (AHSs). We also investigated both the relationship between HI and hemoglobin concentration (Hb) and that between HI and 31 biochemical test values in AHSs. Results In the interference assessment, only HI-4 showed no influence on the 3 interference components. The correlation between Hb and HI-4 was very strong (rS = 0.9987). A 1-unit increase in HI-4 corresponded to a 14.8-mg/dL increase in Hb. Conclusion We found the best wavelength for HI to be at or near 571 nm.
Databáze: OpenAIRE
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