Efficiency of benthic diatom-associated bacteria in the removal of benzo(a)pyrene and fluoranthene

Autor: Manel Ben Abdallah, Asma Sakka Hlaili, Oumayma Kahla, Sondes Melliti Ben Garali, Marc Tedetti, Marianne Quéméneur, Fatma Karray, Olivier Pringault, Hatem Zaghden, Najla Mhiri, Najwa Kallel, Badreddine Barhoumi, Sami Sayadi
Přispěvatelé: Université de Carthage - University of Carthage, Centre de Biotechnologie de Sfax (CBS), Laboratoire des Bioprocédés Environnementaux, LMI COSYS-Med, Environnements et Paléoenvironnements OCéaniques (EPOC), Observatoire aquitain des sciences de l'univers (OASU), Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Institut méditerranéen d'océanologie (MIO), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Bioprocédés Environnementaux, Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE)
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Science of the Total Environment
Science of the Total Environment, 2021, 751, pp.141399. ⟨10.1016/j.scitotenv.2020.141399⟩
Science of the Total Environment, Elsevier, 2021, 751, pp.141399. ⟨10.1016/j.scitotenv.2020.141399⟩
ISSN: 0048-9697
1879-1026
DOI: 10.1016/j.scitotenv.2020.141399⟩
Popis: We investigated the efficiency of a benthic diatom-associated bacteria in removing benzo(a)pyrene (BaP) and fluoranthene (Flt). The diatom, isolated from a PAH-contaminated sediment of the Bizerte Lagoon (Tunisia), was exposed in axenic and non-axenic cultures to PAHs over 7 days. The diversity of the associated bacteria, both attached (AB) and free-living bacteria (FB), was analyzed by the 16S rRNA amplicon sequencing. The diatom, which maintained continuous growth under PAH treatments, was able to accumulate BaP and Flt, with different efficiencies between axenic and non-axenic cultures. Biodegradation, which constituted the main process for PAH elimination, was enhanced in the presence of bacteria, indicating the co-metabolic synergy of microalgae and associated bacteria in removing BaP and Flt. Diatom and bacteria showed different capacities in the degradation of BaP and Flt. Nitzschia sp. harbored bacterial communities with a distinct composition between attached and free-living bacteria. The AB fraction exhibited higher diversity and abundance relative to FB, while the FB fraction contained genera with the known ability of PAH degradation, such as Marivita, Erythrobacter, and Alcaligenes. Moreover, strains of Staphylococcus and Micrococcus, isolated from the FB community, showed the capacity to grow in the presence of crude oil. These results suggest that a “benthic Nitzschia sp.-associated hydrocarbon-degrading bacteria” consortium can be applied in the bioremediation of PAH-contaminated sites. This work was supported by IRD through the Laboratoire Mixte International (LMI) COSYS-Med (Contaminants et Ecosystèmes Sud Méditerranéens). English grammar and syntax of the manuscript were revised by Proof-Reading-Service.com. We thank anonymous reviewers for useful suggestions.
Databáze: OpenAIRE
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