Human coxsackie adenovirus receptor (CAR) expression in transgenic mouse prostate tumors enhances adenoviral delivery of genes
| Autor: | Yunhua Bao, Jiping Chen, Weidan Peng, Lily Wu, Amy Verbitsky, Janet A. Sawicki, Katherine A. Rauen |
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| Rok vydání: | 2005 |
| Předmět: |
Genetically modified mouse
Male Virus genetics Coxsackie and Adenovirus Receptor-Like Membrane Protein Urology Genetic enhancement Transgene Gene Expression Mice Transgenic Biology urologic and male genital diseases medicine.disease_cause Viral vector Adenoviridae Prostate cancer Mice medicine Animals Humans Genetic transfer Prostatic Neoplasms Genetic Therapy medicine.disease Mice Inbred C57BL Disease Models Animal Oncology Immunology Cancer research Receptors Virus |
| Zdroj: | The Prostate. 64(4) |
| ISSN: | 0270-4137 |
| Popis: | BACKGROUND Transgenic mice that recapitulate the progression of human diseases are potentially useful models for testing the effectiveness of new therapeutic strategies. Their use in pre-clinical testing of adenovirally-delivered gene therapies, however, is limited because of restricted cell surface expression of Coxsackie adenovirus receptor (CAR) in mice. METHODS To develop a more suitable transgenic mouse model for testing adenoviral-based gene therapies for prostate cancer, we generated prostate specific antigen/human CAR (PSA/hCAR) transgenic mice in which a chimeric enhancer/promoter sequence of the human PSA gene drives expression of a functional hCAR coding sequence. Results Expression of an adenovirally-delivered luciferase reporter gene in prostate tumor cells in bigenic mice (PSA/hCAR + TRAMP) was enhanced compared to the level in tumor cells lacking the PSA/hCAR transgene. CONCLUSIONS Breeding PSA/hCAR mice to existing transgenic mouse models for prostate cancer (e.g., TRAMP) results in improved mouse models for testing adenovirally-delivered therapeutic genes. © 2005 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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