Indole-3-acetic acid is a physiological inhibitor of TORC1 in yeast
| Autor: | Malika Jaquenoud, Guillermo Miguel Garcia Osuna, Michael Stumpe, Serena Raucci, Agnès H. Michel, Benoît Kornmann, Raffaele Nicastro, Claudio De Virgilio |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Cancer Research Cell division Physiology Fungal Physiology Yeast and Fungal Models Plant Science QH426-470 Biochemistry 01 natural sciences chemistry.chemical_compound Microbial Physiology Mobile Genetic Elements heterocyclic compounds Genetics (clinical) 0303 health sciences Effector Eukaryota food and beverages Genomics Experimental Organism Systems Plant Physiology Research Article Signal Transduction Transposable element Saccharomyces cerevisiae Context (language use) Mycology Mechanistic Target of Rapamycin Complex 1 Biology Research and Analysis Methods Biosynthesis Microbiology Saccharomyces 03 medical and health sciences Model Organisms Genetic Elements Genetics Extracellular Fungal Genetics Protein Kinase Inhibitors Molecular Biology Ecology Evolution Behavior and Systematics 030304 developmental biology Dose-Response Relationship Drug Indoleacetic Acids Organisms Fungi Transposable Elements Biology and Life Sciences biology.organism_classification Yeast Enzyme Activation chemistry Animal Studies DNA Transposable Elements Indole-3-acetic acid 010606 plant biology & botany |
| Zdroj: | PLoS Genetics PLoS Genetics, Vol 17, Iss 3, p e1009414 (2021) |
| ISSN: | 1553-7404 |
| DOI: | 10.1371/journal.pgen.1009414 |
| Popis: | Indole-3-acetic acid (IAA) is the most common, naturally occurring phytohormone that regulates cell division, differentiation, and senescence in plants. The capacity to synthesize IAA is also widespread among plant-associated bacterial and fungal species, which may use IAA as an effector molecule to define their relationships with plants or to coordinate their physiological behavior through cell-cell communication. Fungi, including many species that do not entertain a plant-associated life style, are also able to synthesize IAA, but the physiological role of IAA in these fungi has largely remained enigmatic. Interestingly, in this context, growth of the budding yeast Saccharomyces cerevisiae is sensitive to extracellular IAA. Here, we use a combination of various genetic approaches including chemical-genetic profiling, SAturated Transposon Analysis in Yeast (SATAY), and genetic epistasis analyses to identify the mode-of-action by which IAA inhibits growth in yeast. Surprisingly, these analyses pinpointed the target of rapamycin complex 1 (TORC1), a central regulator of eukaryotic cell growth, as the major growth-limiting target of IAA. Our biochemical analyses further demonstrate that IAA inhibits TORC1 both in vivo and in vitro. Intriguingly, we also show that yeast cells are able to synthesize IAA and specifically accumulate IAA upon entry into stationary phase. Our data therefore suggest that IAA contributes to proper entry of yeast cells into a quiescent state by acting as a metabolic inhibitor of TORC1. Author summary Auxins are a major group of plant phytohormones that are critical for growth and development. Amongst the auxins, indole-3-acetic acid (IAA) is the most common, naturally occurring phytohormone that regulates cell division, differentiation, and senescence in plants. Interestingly, the capacity to synthesize and secrete IAA is also widespread among fungi, including the budding yeast Saccharomyces cerevisiae, but the role of IAA in fungi has largely remained unknown. Here, we confirm an earlier observation that IAA inhibits growth of budding yeast and show by diverse genetic and biochemical means that IAA restrains budding yeast growth by inhibiting the target of rapamycin complex 1 (TORC1), a highly conserved eukaryotic regulator of growth. Intriguingly, budding yeast cells accumulate IAA specifically when limited for nutrients, which suggests that IAA plays a hitherto unknown physiological role in contributing to the establishment of cellular quiescence by acting as a metabolic inhibitor of TORC1. |
| Databáze: | OpenAIRE |
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