A small RNA pathway mediates global allelic dosage in endosperm
| Autor: | Robert M. Erdmann, P. R. V. Satyaki, Mary Gehring, Maja Klosinska |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
Genetics 0303 health sciences Small RNA 050208 finance fungi 05 social sciences digestive oral and skin physiology food and beverages RNA Biology 01 natural sciences Genome Endosperm Transcriptome 03 medical and health sciences genomic DNA 0502 economics and business 050207 economics Paternal Inheritance Gene 030304 developmental biology 010606 plant biology & botany |
| Popis: | SummaryBalance between maternal and paternal genomes within the triploid endosperm is necessary for normal seed development. The majority of genes in Arabidopsis endosperm are expressed in a 2:1 maternal:paternal ratio, reflecting endosperm genomic DNA content. Here we find that the 2:1 transcriptional ratio is not, unexpectedly, a passive default but is actively regulated. We describe an inverse relationship between the parent-of-origin of small RNAs and mRNAs in endosperm on a genome-wide scale. Disruption of the Pol IV small RNA pathway causes the entire transcriptome to become more maternally biased. Furthermore, paternal inheritance of a RNA Pol IV mutation is sufficient to rescue seed abortion caused by excess paternal genome dosage. These results indicate that maintenance of the maternal:paternal transcriptome ratio in endosperm is an active process and reveal a function for RNA Pol IV in mediating the global transcriptional balance between maternally and paternally inherited genomes in endosperm. |
| Databáze: | OpenAIRE |
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