Comparison of the Effects of Ionic and Non-ionic Solutions on the Volume and Intracellular Potassium of Frozen and Non-frozen Human Red Cells
| Autor: | C. R. Valeri, W. T. Sampson, A. H. Runck |
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| Rok vydání: | 1968 |
| Předmět: |
Adult
Glycerol Male Erythrocytes Potassium Hypertonic Solutions Immunology Indicator Dilution Techniques Ionic bonding chemistry.chemical_element Centrifugation Blood Sedimentation Electrolytes chemistry.chemical_compound Freezing medicine Humans Immunology and Allergy Mean corpuscular volume Acid-Base Equilibrium Blood Volume Chromatography Blood Volume Determination medicine.diagnostic_test Chemistry Sodium Anticoagulants Hematology Dilution Volume (thermodynamics) Biochemistry Blood Preservation Tonicity Blood Chemical Analysis |
| Zdroj: | Transfusion. 8:9-18 |
| ISSN: | 1537-2995 0041-1132 |
| DOI: | 10.1111/j.1537-2995.1968.tb02380.x |
| Popis: | Measurements of intracellular potassium and mean corpuscular volume (MCV) were used to evaluate the effects of washing human red cells by either a continuous wash technic with ionic solutions, or a dilution technic with non-electrolyte solutions and recovery by agglomeration. The red cells were either ACD-stored, or preserved in glycerol with or without slow freezing. Significant reduction in cellular potassium was observed when agglomeration was used to recover the red cells, whereas no significant change was observed with washing using continuous centrifugation with ionic solutions. No significant change in mean corpuscular volume of red cells was observed with either technic. The effect of hypertonic ionic and non-ionic washing solutions on intracellular potassium was also evaluated. The data suggest that the removal of glycerol from previously frozen red cells should be accomplished by hypertonic ionic solutions. |
| Databáze: | OpenAIRE |
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