A comparative study of methods for the diagnosis of respiratory virus infections in childhood
| Autor: | Joyce McQuillin, Patricia M. sturdy, P. S. Gardner |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1969 |
| Předmět: |
Rhinovirus
Epidemiology Orthomyxoviridae Fluorescent Antibody Technique Picornaviridae Nose medicine.disease_cause Virus Respirovirus Adenoviridae Culture Techniques medicine Methods Animals Humans Simplexvirus Respiratory Tract Infections biology Respiratory tract infections business.industry Sputum Infant Haplorhini biology.organism_classification Respiratory Syncytial Viruses Infectious Diseases medicine.anatomical_structure Nasal Swab Virus Diseases Child Preschool Immunology Respiratory virus Pharynx medicine.symptom business Research Article |
| Popis: | SUMMARYNasopharyngeal secretions and cough/nasal swabs were taken from 111 children admitted to hospital in Newcastle upon Tyne with acute lower respiratory disease. A comparison was made between nasopharyngeal secretions and cough/nasal swabs as material for isolation of viruses in tissue culture. These results were, in turn, compared with those obtained by applying a fluorescent antibody technique to the exfoliated cells in the nasopharyngeal secretions for the rapid diagnosis of respiratory syncytial virus infection.More viruses were isolated in tissue culture from nasopharyngeal secretion than from cough/nasal swabs. Further evidence for the superiority of nasopharyngeal secretions was obtained by comparing the virus isolations in the laboratory in 1967 with those in 1968. Respiratory syncytial virus was not only isolated more often but more quickly in tissue culture inoculated with nasopharyngeal secretions.The fluorescent antibody technique not only provided a diagnosis on the patient's day of admission in 95% of those infected with respiratory syncytial virus but also proved to be as sensitive as the culture of nasopharyngeal secretions and considerably more sensitive than the culture of cough/nasal swabs for the diagnosis of respiratory syncytial virus infection.We wish to acknowledge the invaluable help given us by consultants, medical officers, ward sisters and their staff in the paediatric wards of the Child Health Department. We also wish to thank the technical staff in the Department of Virology for their aid. |
| Databáze: | OpenAIRE |
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