Engineered mucoperiosteal scaffold for cleft palate regeneration towards the non-immunogenic transplantation
| Autor: | Annapina Palmieri, A. M. Ferranti, M. Cajozzo, Lucia Leone, C. De Stefanis, Franco Locatelli, S. Tedesco, Maria Ida Rizzo, Daniele Mezzogori, G. Pozzato, Mario Zama, M. Esposito, Luigi Tomao, Mattia Algeri |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Scaffold Bone Regeneration Swine Science Settore BIO/09 - FISIOLOGIA Cleft Lip Stem cells Regenerative Medicine Regenerative medicine Collagen Type I Article 03 medical and health sciences 0302 clinical medicine Medical research Engineering Tissue engineering REGENERATION Osteogenesis Medicine Animals Humans Osteonectin Bone regeneration Multidisciplinary Decellularization Tissue Engineering Tissue Scaffolds business.industry Regeneration (biology) SOXB1 Transcription Factors Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Transplantation Cleft Palate Collagen Type I alpha 1 Chain 030104 developmental biology Cellular Microenvironment 030220 oncology & carcinogenesis Tissue Transplantation business Biomedical engineering |
| Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021) |
| ISSN: | 2045-2322 |
| Popis: | Cleft lip and palate (CL/P) is the most prevalent craniofacial birth defect in humans. None of the surgical procedures currently used for CL/P repair lead to definitive correction of hard palate bone interruption. Advances in tissue engineering and regenerative medicine aim to develop new strategies to restore palatal bone interruption by using tissue or organ-decellularized bioscaffolds seeded with host cells. Aim of this study was to set up a new natural scaffold deriving from a decellularized porcine mucoperiosteum, engineered by an innovative micro-perforation procedure based on Quantum Molecular Resonance (QMR) and then subjected to in vitro recellularization with human bone marrow-derived mesenchymal stem cells (hBM-MSCs). Our results demonstrated the efficiency of decellularization treatment gaining a natural, non-immunogenic scaffold with preserved collagen microenvironment that displays a favorable support to hMSC engraftment, spreading and differentiation. Ultrastructural analysis showed that the micro-perforation procedure preserved the collagen mesh, increasing the osteoinductive potential for mesenchymal precursor cells. In conclusion, we developed a novel tissue engineering protocol to obtain a non-immunogenic mucoperiosteal scaffold suitable for allogenic transplantation and CL/P repair. The innovative micro-perforation procedure improving hMSC osteogenic differentiation potentially impacts for enhanced palatal bone regeneration leading to future clinical applications in humans. |
| Databáze: | OpenAIRE |
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