Detection of Pseudomonas aeruginosa in water samples using a novel synthetic medium and impedimetric technology
| Autor: | L. Soos, S. Bernáth, Gy. Csikó, M. Gyenes, L. Békési, T. Rétfalvi, Géza Szita |
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| Rok vydání: | 2007 |
| Předmět: |
Bacteriological Techniques
Chromatography Chemistry Pseudomonas aeruginosa Focused Impedance Measurement Fresh Water Contamination medicine.disease_cause Selective isolation Applied Microbiology and Biotechnology Culture Media Microbiology Swimming Pools Fully automated Acetamides Electric Impedance medicine Water Microbiology Nitrogen source Dialysis Analysis method Environmental Monitoring |
| Zdroj: | Letters in Applied Microbiology. 45:42-46 |
| ISSN: | 1472-765X 0266-8254 |
| Popis: | Aims: To investigate whether the use of a novel synthetic medium in conjunction with impedimetric technology could provide a rapid and automated detection of Pseudomonas aeruginosa in water samples. Methods and Results: A selective synthetic medium (Z-broth) in which the only carbon and nitrogen source is acetamide was applied in direct impedimetric examination for the selective isolation of P. aeruginosa. A total of 1036 tap-water, well-water, swimming-pool water and dialysis water samples were investigated, and any P. aeruginosa contamination was detected in 7–24 h. Neither false-negative nor false-positive results were observed. Conclusions: The results of the present evaluation demonstrate that impedance measurement with the use of Z-broth is suitable for the rapid and automatic detection of P. aeruginosa in water. Significance and Impact of the Study: The main advantages of the method: 240 samples can be examined in one step, the procedure is fully automated, the results are obtained quickly and the labour and media requirements are low. |
| Databáze: | OpenAIRE |
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