Genome-wide analysis of alternative splicing differences between oocyte and zygote†
| Autor: | Xu Liu, Rui Cheng, Chuan Zhou, Mengyun Wang, Yingmei Wang, Yong Zhang, Xiaoman Zheng, Fusheng Quan, Jun Liu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
0106 biological sciences 0301 basic medicine Zygote Embryonic Development Biology Primary transcript 01 natural sciences Genome Mice 03 medical and health sciences Human fertilization Transcription (biology) medicine Animals Protein Isoforms RNA Messenger Gene Alternative splicing Cell Biology General Medicine Embryo Mammalian Oocyte Cell biology Mice Inbred C57BL Alternative Splicing 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Reproductive Medicine Oocytes Female Genome-Wide Association Study 010606 plant biology & botany |
| Zdroj: | Biology of Reproduction. 102:999-1010 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1093/biolre/ioaa004 |
| Popis: | Alternative splicing (AS) of mRNA precursors allows the synthesis of multiple mRNAs from a single primary transcript, significantly expanding the information content and regulatory possibilities of higher eukaryotic genomes. During mammalian development, AS drives certain decisive changes in different physiological processes. As development progresses, the maternal-to-zygotic transition (MZT) will trigger two processes: elimination of a subset of maternal mRNA and transcription of the zygote genome begins. Recent high-throughput technological advancements have facilitated genome-wide AS, whereas its analysis in mouse oocyte transition to the zygote stage has not been reported. We present a high-resolution global analysis of AS transitions and discovered extensive AS transitions between mouse oocyte and zygote. The difference of AS patterns was further confirmed using reverse transcription-polymerase chain reaction analysis. Many genes with specific AS events in mouse oocytes are differentially expressed between oocyte and zygote, but only a few genes with specific AS events in zygote are differentially expressed between oocyte and zygote. We provide a landscape of AS events in mouse oocyte and zygote. Our results advance the understanding of AS transitions during mouse fertilization and its potential functions for MZT and further development. |
| Databáze: | OpenAIRE |
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