Retroviral insertional mutagenesis implicates E3 ubiquitin ligase RNF168 in the control of cell proliferation and survival
| Autor: | Gwyn T. Williams, Joop Gaken, Nicola J. McCarthy, Kiren Yacqub-Usman, David Darling, Farzin Farzaneh, Mark R. Pickard, Shu Uin Gan, Aytug Kizilors, Cathleen E Schulte |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
DNA repair Biophysics Apoptosis Cell fate determination Biochemistry Cell survival Insertional mutagenesis 03 medical and health sciences Ubiquitin Journal Article Progenitor cell Molecular Biology Research Articles Cell proliferation biology Cell growth Cell Biology Forward genetics Ubiquitin ligase Cell biology 030104 developmental biology biology.protein Retroviral Insertional Mutagenesis Research Article QD415 |
| Zdroj: | Bioscience Reports Kizilors, A, Pickard, M R, Schulte, C E, Yacqub-Usman, K, McCarthy, N J, Gan, S-U, Darling, D, Gäken, J, Williams, G T & Farzaneh, F 2017, ' Retroviral insertional mutagenesis implicates E3 ubiquitin ligase RNF168 in the control of cell proliferation and survival ', Bioscience Reports, vol. 37, no. 4, BSR20170843 . https://doi.org/10.1042/BSR20170843 |
| ISSN: | 0144-8463 |
| Popis: | The E3 ubiquitin ligase RNF168 is a ring finger protein that has been previously identified to play an important regulatory role in the repair of double-strand DNA breaks. In the present study, an unbiased forward genetics functional screen in mouse granulocyte/macrophage progenitor cell line FDCP1 has identified E3 ubiquitin ligase RNF168 as a key regulator of cell survival and proliferation. Our data indicate that RNF168 is an important component of the mechanisms controlling cell fate, not only in human and mouse haematopoietic growth factor dependent cells, but also in the human breast epithelial cell line MCF-7. These observations therefore suggest that RNF168 provides a connection to key pathways controlling cell fate, potentially through interaction with PML nuclear bodies and/or epigenetic control of gene expression. Our study is the first to demonstrate a critical role for RNF168 in the mechanisms regulating cell proliferation and survival, in addition to its well-established role in DNA repair. |
| Databáze: | OpenAIRE |
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