Cell Docking, Movement and Cell-Cell Interactions of Heterogeneous Cell Suspensions in a Cell Manipulation Microdevice
| Autor: | Shiaw-Min Hwang, Huang Long-Sun, Fei-Lung Lai, Yu-Hung Wang, Yu-Wei Chung |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Cytotoxicity
Immunologic Cell Population microfluidics Cell Communication Biology lcsh:Chemical technology Biochemistry Article Analytical Chemistry Immune system Cell–cell interaction Suspensions Natural Killer cells Cell Movement Neoplasms cell-cell interaction medicine Pressure Humans microchannel lcsh:TP1-1185 Electrical and Electronic Engineering Particle Size education Cytotoxicity Instrumentation Cell Aggregation education.field_of_study Large cell MEMS cytotoxicity Microfluidic Analytical Techniques medicine.disease Atomic and Molecular Physics and Optics Cell biology Killer Cells Natural Leukemia medicine.anatomical_structure Cancer cell K562 Cells Rheology |
| Zdroj: | Sensors, Vol 11, Iss 10, Pp 9613-9627 (2011) Sensors (Basel, Switzerland) Sensors; Volume 11; Issue 10; Pages: 9613-9627 |
| ISSN: | 1424-8220 |
| Popis: | This study demonstrates a novel cell manipulation microdevice for cell docking, culturing, cell-cell contact and interaction by microfluidic manipulation of heterogeneous cell suspensions. Heterogeneous cell suspensions include disparate blood cells of natural killer cells and leukemia cancer cells for immune cell transplantation therapy. However, NK cell alloreactivity from different healthy donors present various recovery response levels. Little is still known about the interactions and cytotoxicity effects between donor NK cells and recipient cancer cells. The cell-based micro device first showed the capability of cell docking, movement, contact and cell-cell interaction with respect to cell cytotoxicity of NK cells against cancer cells. With various flow tests for live cell loading, flow rates of 10 μL/h were chosen for injection in the central and side flows such that both types of suspension cells could be gently docked at the gap structure in a reaction zone. The trapping number of particles and cells was linearly proportional to the gap length. Finally, the cytotoxicity of around 40% was found to be similar in the case of dilute cells and a large cell population. As a result, the cell manipulation microdevice has been validated for live suspensions of natural killer and cancer cells, and exhibited the capability to measure the cytotoxicity of dilute cell suspensions. |
| Databáze: | OpenAIRE |
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