Increased xanthine oxidase-related ROS production and TRPV1 synthesis preceding DOMS post-eccentric exercise in rats

Autor: Mauro Eduardo Porto da Silveira Junior, Guilherme L. Busanello, Luiz Fernando Freire Royes, Frederico Diniz Lima, Ana Flávia Furian, Mauro Schneider Oliveira, Michele Rechia Fighera, Ana Cláudia Monteiro Braga, Guilherme Bresciani, Pietro Maria Chagas, Cristina W. Nogueira, Leandro Thies Retamoso, Leandro R Ribeiro
Rok vydání: 2016
Předmět:
Male
0301 basic medicine
Xanthine Oxidase
medicine.medical_specialty
Physical Exertion
TRPV Cation Channels
Calcium-Transporting ATPases
medicine.disease_cause
Bioinformatics
Antioxidants
General Biochemistry
Genetics and Molecular Biology
Running
Protein Carbonylation
03 medical and health sciences
chemistry.chemical_compound
Gastrocnemius muscle
0302 clinical medicine
Internal medicine
Delayed onset muscle soreness
medicine
Animals
Eccentric
Rats
Wistar
General Pharmacology
Toxicology and Pharmaceutics
Treadmill
Muscle
Skeletal
Xanthine oxidase
Hand Strength
business.industry
Myalgia
General Medicine
Xanthine
Rats
Uric Acid
030104 developmental biology
Endocrinology
chemistry
Hyperalgesia
Uric acid
medicine.symptom
Reactive Oxygen Species
business
030217 neurology & neurosurgery
Oxidative stress
Zdroj: Life Sciences. 152:52-59
ISSN: 0024-3205
Popis: Aims It is well-known that unaccustomed exercise, especially eccentric exercise, is associated to delayed onset muscle soreness (DOMS). Whether DOMS is associated with reactive oxygen species (ROS) and the transient receptor potential vanilloid 1 (TRPV1) is still an open question. Thus, the aim of this study was to investigate the association between TRPV1 and xanthine oxidase-related ROS production in muscle and DOMS after a bout of eccentric exercise. Main methods Male Wistar rats performed a downhill running exercise on a treadmill at a -16° tilt and a constant speed for 90min (5min/bout separated by 2min of rest). Mechanical allodynia and grip force tests were performed before and 1, 3, 6, 9, 12, 24, 48 and 72h after the downhill running. Biochemical assays probing oxidative stress, purine degradation, xanthine oxidase activity, Ca(2+) ATPase activity and TRPV1 protein content were performed in gastrocnemius muscle at 12, 24, and 48h after the downhill running. Key findings Our statistical analysis showed an increase in mechanical allodynia and a loss of strength after the downhill running. Similarly, an increase in carbonyl, xanthine oxidase activity, uric acid levels and TRPV1 immunoreactivity were found 12h post-exercise. On the other hand, Ca(2+) ATPase activity decreased in all analyzed times. Significance Our results suggest that a possible relationship between xanthine oxidase-related ROS and TRPV1 may exist during the events preceding eccentric exercise-related DOMS.
Databáze: OpenAIRE
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