Polyshrink™ based microfluidic chips and protein microarrays

Autor: Céline A. Mandon, Christophe A. Marquette, Loïc J. Blum, Kevin A. Heyries
Přispěvatelé: Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2010
Předmět:
MESH: Microelectrodes
02 engineering and technology
01 natural sciences
MESH: Antibodies
Monoclonal
chemistry.chemical_compound
MESH: Ink
Electrochemistry
MESH: Animals
MESH: Proteins
Biochip
Immunoassay
Spots
MESH: Protein Array Analysis
Antibodies
Monoclonal
MESH: Polystyrenes
Serum Albumin
Bovine
Equipment Design
General Medicine
Microfluidic Analytical Techniques
021001 nanoscience & nanotechnology
MESH: Cattle
Biochip
Cell adhesion
Protein microarray
Microfluidic
Serology
MESH: Microfluidic Analytical Techniques
Protein microarray
Printing
Ink
0210 nano-technology
MESH: Immunoassay
Biotechnology
Materials science
MESH: Chemiluminescent Measurements
Microfluidics
Protein Array Analysis
Biomedical Engineering
Biophysics
Nanotechnology
Miniaturization
Animals
Humans
MESH: Printing
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biochemistry [q-bio.BM]
MESH: Humans
Inkwell
010401 analytical chemistry
Proteins
0104 chemical sciences
MESH: Hela Cells
chemistry
Luminescent Measurements
Polystyrenes
Cattle
Polystyrene
Microelectrodes
MESH: Serum Albumin
Bovine
HeLa Cells
MESH: Equipment Design
Zdroj: Biosensors and Bioelectronics
Biosensors and Bioelectronics, Elsevier, 2010, 26 (4), pp.1218-24. ⟨10.1016/j.bios.2010.05.028⟩
ISSN: 0956-5663
DOI: 10.1016/j.bios.2010.05.028
Popis: International audience; A new approach for the rapid production of microfluidic chips integrating protein spots is described. The technology, called "Print-n-Shrink", is based on the screen-printing of a microfluidic design (using a dielectric ink) onto Polyshrink™ polystyrene sheets. The initial printing which have a minimum size of 15 μm (height)×230 μm (width) was thermally treated (30s, 163°C) to shrink and generate features of 85 μm (height)×100 μm (width). Protein spots were also demonstrated to be shrinkable and arrays of 50 μm-size spots with density up to 6400 spots/cm(2) were achieved. Proteins such as monoclonal antibodies or cellular adhesion proteins were thus spotted onto the Polyshrink™ sheets and shrunk together with the microfluidic design, creating complete biochips integrating both complex microfluidic designs and protein spots for bioanalytical applications. These shrunk spots were shown to host enough active proteins to enable the achievement of both sensitive sandwich immunoassays (Brain Natriuretic Peptide, C-Reactive Protein and c-Troponin I) and localized cell culture.
Databáze: OpenAIRE
Externí odkaz: