Fabrication of microtemplates for the control of bacterial immobilization
| Autor: | Koji Mitamura, Osamu Takai, Yasuhiro Miyahara, Nagahiro Saito |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Materials science
technology industry and agriculture Nanotechnology Self-assembled monolayer Surfaces and Interfaces Chemical vapor deposition Condensed Matter Physics Surfaces Coatings and Films chemistry.chemical_compound chemistry Superhydrophilicity Monolayer PEG ratio Wetting Self-assembly Ethylene glycol |
| Zdroj: | Journal of Vacuum Science & Technology A: Vacuum, Surfaces, and Films. 27:1183-1187 |
| ISSN: | 1520-8559 0734-2101 |
| Popis: | The authors described a region-selective immobilization methods of bacteria by using superhydrophobic/superhydrophilic and superhydrophobic/poly(ethylene glycol) (PEG) micropatterns for culture scaffold templates. In the case of superhydrophobic/superhydrophilic micropatterns, the superhydrophobic surface was prepared first by microwave-plasma enhanced chemical vapor deposition (MPECVD) from trimethylmethoxysilane. Then the superhydrophilic regions were fabricated by irradiating the superhydrophobic surface with vuv light through a stencil mask. In the case of the superhydrophobic/PEG micropatterned surfaces, PEG surfaces were fabricated first by chemical reaction of ester groups of p-nitrophenyl PEG with NH2 group of NH2-terminated self assembled monolayer from n-6-hexyl-3-aminopropyltrimethoxysilane. The superhydrophobic regions were fabricated by MPECVD thorough a stencil mask. In this study four bacteria were selected from viewpoint of peptidoglycan cell wall (E. coli versus B. subtilis), extracellular polysaccharide (E.coli versus P. stutzeri, P. aeruginosa), and growth rate (P. stutzeri versus P. aeruginosa). The former micropattern brought discrete adhesions of E. coli and B. subtilis specifically on the hydrophobic regions, Furthermore, using the superhydrophobic/PEG micropattern, adhesion of bacteria expanded for E. coli, B. subtilis, P. stutzeri, and P. aeruginosa. They observed a high bacterial adhesion onto superhydrophobic surfaces and the inhibitive effect of bacterial adhesion on PEG surfaces. |
| Databáze: | OpenAIRE |
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