Two genes encoding the soybean translation elongation factor eEF-1? are transcribed in seedling leaves
| Autor: | Paul-Etienne Montandon, Fernando Aguilar, Erhard Stutz |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Transcription
Genetic Ribulose-Bisphosphate Carboxylase Molecular Sequence Data Plant Science Protein Sorting Signals Biology eEF-1 Peptide Elongation Factor 1 Transcription (biology) Complementary DNA Genetics Amino Acid Sequence Cloning Molecular Gene Plant Proteins Genomic Library Base Sequence Oligonucleotide Nucleic acid sequence Intron DNA Exons General Medicine Blotting Northern Peptide Elongation Factors Molecular biology Introns Blotting Southern genomic DNA Seeds Autoradiography RNA Soybeans Agronomy and Crop Science |
| Zdroj: | Plant Molecular Biology. 17:351-360 |
| ISSN: | 1573-5028 0167-4412 |
| DOI: | 10.1007/bf00040630 |
| Popis: | A cDNA and a genomic DNA library from soybean (Glycine max L.) were used to identify and sequence two genes coding for the alpha-subunit of the translation elongation factor eEF-1. Within the coding part, the two genes (tefS1 and tefS2) diverge in 80 wobble positions thus yielding an identical protein composed of 447 amino acids. The soybean protein has about 95% similarity with eEF-1 alpha proteins of Arabidopsis thaliana and tomato. Both genes S1 and S2 contain, within the coding part at a site seemingly unique to higher plants, a single short intron of 86 and 116 nucleotides, respectively. The untranslated leader part of both genes is interrupted by a large intron (partially sequenced). Genes S1 and S2 are transcribed in young leaves. cDNA and gene-specific oligonucleotide probes interact with a unique transcript of close to 1.9 kb. Northern hybridization studies using RNAs from dark- and light-grown seedlings show that light sharply increases the level of stable transcripts (1.9 kb). A peak value is measured after about 3 h of illumination, afterwards the transcript concentration drops to about 10% of the peak value. Genes S1 and S2 follow a similar transcription pattern in developing seedling leaves, which is distinct from that of the rbcS genes measured in parallel experiments. According to northern results, S1 transcripts are more abundant in leaves at all measured stages of development than S2 transcripts. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink