Human Small Cell Lung Cancer Cells Produce Brain Natriuretic Peptide
| Autor: | Herbert K. Oie, BruceE. Johnson, Yoshinobu Ohsaki, Phuong Tram Le, Andrew J. Gross |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Lung Neoplasms medicine.drug_class Radioimmunoassay Biology Peptide hormone Polymerase Chain Reaction Small-cell carcinoma Pathogenesis Natriuretic Peptide Brain Tumor Cells Cultured medicine Carcinoma Natriuretic peptide Humans RNA Messenger RNA Neoplasm cardiovascular diseases Carcinoma Small Cell DNA Primers Respiratory disease DNA Neoplasm General Medicine respiratory system medicine.disease Brain natriuretic peptide respiratory tract diseases Gene Expression Regulation Neoplastic Oncology Cell culture human activities hormones hormone substitutes and hormone antagonists |
| Zdroj: | Oncology. 56:155-159 |
| ISSN: | 1423-0232 0030-2414 |
| DOI: | 10.1159/000011957 |
| Popis: | The tumoral production of brain natriuretic peptide (BNP) was studied using 9 small cell lung cancer (SCLC) cell lines which were established from patients with small cell lung cancer. BNP cDNA fragment was generated from 20 µg total RNA which was prepared from the human right cardiac atrium by reverse transcription-based polymerase chain reaction. Expression of BNP mRNA was detected in 30 µg total cellular RNA from these cell lines by RNase protection assays in 5 of 9 SCLC cell lines. Radioimmunoassays using 125I-radiolabeled human BNP(1–32) and antihuman BNP(1–32) antibody detected immunoreactivity in cell pellets from SCLC cell lines which had detectable BNP mRNA. BNP immunoreactivity in the cell pellets corresponds with the data from BNP mRNA analyses. We conclude that SCLC cells have detectable BNP mRNA by RNase protection assay and BNP immunoreactivity in the cells. |
| Databáze: | OpenAIRE |
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