Discrepant Results of Experimental Human Mesenchymal Stromal Cell Therapy after Myocardial Infarction: Are Animal Models Robust Enough?
| Autor: | Niek J. Pluijmert, Vanessa-leigh van Zuylen, Cindy I. Schutte, Helene Roelofs, Martin J. Schalij, Melina C. den Haan, Douwe E. Atsma, Willem E. Fibbe |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pathology Physiology medicine.medical_treatment Cell- and Tissue-Based Therapy Myocardial Infarction lcsh:Medicine Mice SCID 030204 cardiovascular system & hematology Cardiovascular Physiology Biochemistry Diagnostic Radiology Mice Spectrum Analysis Techniques 0302 clinical medicine Mice Inbred NOD Animal Cells Medicine and Health Sciences Myocardial infarction lcsh:Science Multidisciplinary medicine.diagnostic_test Stem Cells Radiology and Imaging Heart Animal Models Flow Cytometry Magnetic Resonance Imaging Cardiovascular physiology Cytokine Spectrophotometry Cytophotometry Cellular Types Anatomy Research Article Cardiac function curve medicine.medical_specialty Stromal cell Imaging Techniques Cardiology Mouse Models Research and Analysis Methods 03 medical and health sciences Model Organisms Diagnostic Medicine medicine Animals Humans business.industry lcsh:R Mesenchymal stem cell Biology and Life Sciences Proteins Mesenchymal Stem Cells Magnetic resonance imaging Cell Biology medicine.disease Disease Models Animal 030104 developmental biology Cardiovascular Anatomy lcsh:Q Interferons business Ligation |
| Zdroj: | PLoS ONE PLoS ONE, 11(4) PLoS ONE, Vol 11, Iss 4, p e0152938 (2016) |
| ISSN: | 1932-6203 |
| Popis: | Background Human mesenchymal stromal cells (MSCs) have been reported to preserve cardiac function in myocardial infarction (MI) models. Previously, we found a beneficial effect of intramyocardial injection of unstimulated human MSCs (uMSCs) on cardiac function after permanent coronary artery ligation. In the present study we aimed to extend this research by investigating the effect of intramyocardial injection of human MSCs pre-stimulated with the pro-inflammatory cytokine interferon-gamma (iMSCs), since pro-inflammatory priming has shown additional salutary effects in multiple experimental disease models. Methods MI was induced in NOD/Scid mice by permanent ligation of the left anterior descending coronary artery. Animals received intramyocardial injection of uMSCs, iMSCs or PBS. Sham-operated animals were used to determine baseline characteristics. Cardiac performance was assessed after 2 and 14 days using 7-Tesla magnetic resonance imaging and pressure-volume loop measurements. Histology and q-PCR were used to confirm MSC engraftment in the heart. Results Both uMSC and iMSC therapy had no significant beneficial effect on cardiac function or remodelling in contrast to our previous studies. Conclusions Animal models for cardiac MSC therapy appear less robust than initially envisioned. |
| Databáze: | OpenAIRE |
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