Early postnatal feed restriction reduces liver connective tissue levels and affects H3K9 acetylation state of regulated genes associated with protein metabolism in low birth weight pigs
| Autor: | Gerald Rimbach, Ricarda Krüger, Elke Albrecht, Cornelia C. Metges, Karen Martens, Solvig Görs, Katrin Giller, C. Nebendahl, Harald M. Hammon |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty XBP1 Transcription Genetic Swine Endocrinology Diabetes and Metabolism Birth weight Clinical Biochemistry Protein metabolism Connective tissue Protein degradation Biology Biochemistry Histones 03 medical and health sciences chemistry.chemical_compound Internal medicine Gene expression medicine Animals Birth Weight Epigenetics Molecular Biology Caloric Restriction Nutrition and Dietetics Gene Expression Profiling Proteins Acetylation Animal Feed Low birth weight 030104 developmental biology Endocrinology medicine.anatomical_structure Gene Expression Regulation Liver chemistry Connective Tissue Immunology medicine.symptom |
| Zdroj: | The Journal of Nutritional Biochemistry. 29:41-55 |
| ISSN: | 0955-2863 |
| Popis: | Intrauterine growth retardation is associated with metabolic consequences in adulthood. Since our previous data indicate birth weight-dependent effects of feed restriction (R) on protein degradation processes in the liver, it should be investigated whether effects on connective tissue turnover are obvious and could be explained by global changes of histone H3K9me3 and H3K9ac states in regulated genes. For this purpose, female littermate pigs with low (U) or normal (N) birth weight were subjected to 3-week R (60% of ad libitum fed controls) with subsequent refeeding (REF) for further 5 weeks. The 3-week R-period induced a significant reduction of connective tissue area by 43% in the liver of U animals at 98 d of age, which was not found in age-matched N animals. Of note, after REF at 131 d of age, in previously feed-restricted U animals (UR), the percentage of mean connective tissue was only 53% of ad libitum fed controls (UK), indicating a persistent effect. In U animals, R induced H3K9 acetylation of regulated genes (e.g. XBP1, ERLEC1, GALNT2, PTRH2), which were inter alia associated with protein metabolism. In contrast, REF was mostly accompanied by deacetylation in U and N animals. Thus, our epigenetic data may give a first explanation for the observed birth weight-dependent differences in this connective tissue phenotype. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect