A DNAzyme-mediated target-initiated rolling circle amplification strategy based on a microchip platform for the detection of apurinic/apyrimidinic endonuclease 1 at the single-cell level

Autor:	Xiaoshu Luo, Chunhuan Xu, Shulin Zhao, Jingjin Zhao, Shengyu Chen
Rok vydání:	2021
Předmět:	Electrophoresis Lysis Deoxyribozyme Biosensing Techniques Cellular level Catalysis HeLa Endonuclease Limit of Detection Materials Chemistry Humans AP site Apurinic apyrimidinic endonuclease biology Chemistry Metals and Alloys General Chemistry DNA Catalytic biology.organism_classification Endonucleases Microarray Analysis Molecular biology Surfaces Coatings and Films Electronic Optical and Magnetic Materials Rolling circle replication Ceramics and Composites biology.protein MCF-7 Cells Single-Cell Analysis Nucleic Acid Amplification Techniques HeLa Cells
Zdroj:	Chemical communications (Cambridge, England). 57(84)
ISSN:	1364-548X
Popis:	A DNAzyme-mediated target-initiated rolling circle signal amplification strategy based on a microchip platform was developed for detecting apurinic/apyrimidine endonuclease 1 (APE1) at the single-cell level. This strategy was applied to assays of lysate samples from HL-7702, HeLa and MCF-7 cells, with a detection limit of lower than 1 HeLa cell.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d812caad80a4650c03b7ec1d92123316 https://pubmed.ncbi.nlm.nih.gov/34605511 Zobrazit plný text záznamu