Squalene-PEG: Pyropheophorbide-a nanoconstructs for tumor theranostics

Autor: Gregor Trefalt, Souad Adriouach, Andrej Babič, Norbert Lange, Eric Allémann, Vassily Vorobiev
Rok vydání: 2019
Předmět:
Chlorophyll
Male
Squalene
Light
Biocompatibility
medicine.medical_treatment
Biomedical Engineering
Pharmaceutical Science
Medicine (miscellaneous)
Apoptosis
Bioengineering
Photodynamic therapy
Chick Embryo
02 engineering and technology
Chorioallantoic Membrane
Theranostic Nanomedicine
Polyethylene Glycols
Mice
03 medical and health sciences
chemistry.chemical_compound
Drug Delivery Systems
In vivo
PEG ratio
Tumor Cells
Cultured
medicine
Animals
Humans
General Materials Science
Photosensitizer
Cell Proliferation
030304 developmental biology
0303 health sciences
Photosensitizing Agents
Prostatic Neoplasms
respiratory system
021001 nanoscience & nanotechnology
Xenograft Model Antitumor Assays
Photochemotherapy
chemistry
Drug delivery
Biophysics
Nanoparticles
Molecular Medicine
Female
Glioblastoma
0210 nano-technology
Phototoxicity
Zdroj: Nanomedicine: Nanotechnology, Biology and Medicine. 15:243-251
ISSN: 1549-9634
Popis: Novel nanoscale drug delivery biomaterials are of great importance for the diagnosis and treatment of different cancers. We have developed a new pegylated squalene (SQ-PEG) derivative with self-assembly properties. Supramolecular assembly with a lipophilic photosensitizer pyropheophorbide-a (Ppa) by nanoprecipitation gave nanoconstructs SQ-PEG:Ppa with an average size of 200 nm in diameter and a drug loading of 18% (w/w). The composite material demonstrates nanoscale optical properties by tight packing of Ppa within Sq-PEG:Ppa resulting in 99.99% fluorescence self-quenching. The biocompatibility of the nanomaterial and cell phototoxicity under light irradiation were investigated on PC3 prostate tumor cells in vitro. SQ-PEG:Ppa showed excellent phototoxic effect at low light dose of 5.0 J/cm2 as a consequence of efficient cell internalization of Ppa by the nanodelivery system. The diagnostic potential of SQ-PEG:Ppa nanoconstructs to deliver Ppa to tumors in vivo was demonstrated in chick embryo model implanted with U87MG glioblastoma micro tumors.
Databáze: OpenAIRE
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