Comprehensive Analysis of Antibodies Induced by Vaccination with 4 Kinds of Avian Influenza H5N1 Pre-Pandemic Vaccines

Autor: Keiko Majima, Yoshitaka Iba, Daisuke Hirano, Ritsuko Kubota-Koketsu, Yoshinobu Okuno, Ayami Yamasaki, Yoshikazu Kurosawa, Nobuko Ohshima, Mototaka Sugiura, Shunji Yoshida, Yoshizo Asano, Gene Kurosawa
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Male
0301 basic medicine
Antibodies
Viral
medicine.disease_cause
lcsh:Chemistry
pre-pandemic vaccines
Influenza A Virus
H1N1 Subtype
0302 clinical medicine
Japan
Pandemic
antibodies
lcsh:QH301-705.5
Spectroscopy
B-Lymphocytes
Vaccination
General Medicine
H5N1
Middle Aged
Healthy Volunteers
Computer Science Applications
Influenza Vaccines
Female
Antibody
Adult
H5N1 vaccine
Cross Reactions
Biology
Article
Catalysis
Virus
Inorganic Chemistry
03 medical and health sciences
Influenza
Human
medicine
Humans
Physical and Theoretical Chemistry
Pandemics
Molecular Biology
Aged
Hemagglutination assay
Influenza A Virus
H5N1 Subtype
Organic Chemistry
Hemagglutination Inhibition Tests
Virology
Influenza A virus subtype H5N1
030104 developmental biology
Vaccines
Inactivated
lcsh:Biology (General)
lcsh:QD1-999
Immunization
biology.protein
Immunologic Memory
Broadly Neutralizing Antibodies
030215 immunology
Zdroj: International Journal of Molecular Sciences
Volume 21
Issue 19
International Journal of Molecular Sciences, Vol 21, Iss 7422, p 7422 (2020)
ISSN: 1422-0067
DOI: 10.3390/ijms21197422
Popis: Four kinds of avian-derived H5N1 influenza virus, A/Vietnam/1194/2004 (Clade 1), A/Indonesia/5/2005 (Clade 2.1), A/Qinghai/1A/2005 (Clade 2.2), and A/Anhui/1/2005 (Clade 2.3), have been stocked in Japan for use as pre-pandemic vaccines. When a pandemic occurs, these viruses would be used as vaccines in the hope of inducing immunity against the pandemic virus. We analyzed the specificity of antibodies (Abs) produced by B lymphocytes present in the blood after immunization with these vaccines. Eighteen volunteers took part in this project. After libraries of Ab-encoding sequences were constructed using blood from subjects vaccinated with these viruses, a large number of clones that encoded Abs that bound to the virus particles used as vaccines were isolated. These clones were classified into two groups according to the hemagglutination inhibition (HI) activity of the encoded Abs. While two-thirds of the clones were HI positive, the encoded Abs exhibited only restricted strain specificity. On the other hand, half of the HI-negative clones encoded Abs that bound not only to the H5N1 virus but also to the H1N1 virus
with a few exceptions, these Abs appeared to be encoded by memory B cells present before vaccination. The HI-negative clones included those encoding broadly cross-reactive Abs, some of which were encoded by non-VH1-69 germline genes. However, although this work shows that various kinds of anti-H5N1 Abs are encoded by volunteers vaccinated with pre-pandemic vaccines, broad cross-reactivity was seen only in a minority of clones, raising concern regarding the utility of these H5N1 vaccine viruses for the prevention of H5N1 pandemics.
Databáze: OpenAIRE
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