In Vivo Transmigrated Human Neutrophils Are Highly Primed for Intracellular Radical Production Induced by Monosodium Urate Crystals
| Autor: | Karin Christenson, Felix Peter Sanchez Klose, Lisa Davidsson, Johan Bylund, Alicia Buck, Agnes Dahlstrand Rudin, Lena Björkman |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Adult
Gout Neutrophils neutrophil extracellular traps MPO Inflammation Extracellular Traps Catalysis Article lcsh:Chemistry Inorganic Chemistry Chronic granulomatous disease In vivo medicine Humans Physical and Theoretical Chemistry lcsh:QH301-705.5 Molecular Biology Spectroscopy Cells Cultured Aged Peroxidase NADPH oxidase biology Chemistry Organic Chemistry Transendothelial and Transepithelial Migration NETs General Medicine Neutrophil extracellular traps granule Middle Aged medicine.disease Molecular biology Computer Science Applications Respiratory burst Uric Acid myeloperoxidase lcsh:Biology (General) lcsh:QD1-999 inflammation Myeloperoxidase biology.protein Female medicine.symptom Reactive Oxygen Species Intracellular NADPH-oxidase |
| Zdroj: | International Journal of Molecular Sciences Volume 21 Issue 11 International Journal of Molecular Sciences, Vol 21, Iss 3750, p 3750 (2020) |
| ISSN: | 1422-0067 |
| Popis: | Gout is an inflammatory disease caused by monosodium urate (MSU) crystals. The role of neutrophils in gout is less clear, although several studies have shown neutrophil extracellular trap (NET) formation in acutely inflamed joints of gout patients. MSU crystals are known to induce the production of reactive oxygen species (ROS) and NET formation in neutrophils isolated from blood, but there is inconclusive knowledge on the localization of ROS production as well as whether the ROS are required for NET formation. In this report we demonstrate that MSU crystals activate human neutrophils to produce ROS exclusively in intracellular compartments. Additionally, in vivo transmigrated neutrophils derived from experimental skin chambers displayed markedly increased ROS production as compared to resting blood neutrophils. We also confirmed that MSU stimulation potently induced NET formation, but this response was not primed in in vivo transmigrated neutrophils. In line with this we found that MSU-triggered NET formation was independent of ROS production and proceeded normally in neutrophils from patients with dysfunctional respiratory burst (chronic granulomatous disease (CGD) and complete myeloperoxidase (MPO) deficiency). Our data indicate that in vivo transmigrated neutrophils are markedly primed for oxidative responses to MSU crystals and that MSU triggered NET formation is independent of ROS production. |
| Databáze: | OpenAIRE |
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