Robust Production of Uniform Human Cerebral Organoids from Pluripotent Stem Cells
| Autor: | J. Javier Hernandez, Parisa Ghiasi, Adam Sivitilli, Jean-Martin Beaulieu, Jeffrey L. Wrana, Alesya Evstratova, Daniel Trcka, Liliana Attisano, Jessica Gosio, Bibaswan Ghoshal |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Pluripotent Stem Cells Cell type Brain development Health Toxicology and Mutagenesis Neurogenesis Cell Induced Pluripotent Stem Cells Cell Culture Techniques Plant Science Immunofluorescence Microscopy Biology Biochemistry Genetics and Molecular Biology (miscellaneous) 03 medical and health sciences 0302 clinical medicine Neural Stem Cells medicine Organoid Methods Humans Production pipeline Induced pluripotent stem cell 030304 developmental biology 0303 health sciences Ecology Brain Cell Differentiation Stem cell compartment Cell biology Organoids 030104 developmental biology medicine.anatomical_structure Female 030217 neurology & neurosurgery Cerebral organoid |
| Zdroj: | Life Science Alliance |
| DOI: | 10.1101/2020.03.08.979013 |
| Popis: | By leveraging advances in 3D stem cell culture techniques, the authors describe, characterize and validate a novel platform to efficiently generate morphologically consistent human forebrain cerebral organoids. Human cerebral organoid (hCO) models offer the opportunity to understand fundamental processes underlying human-specific cortical development and pathophysiology in an experimentally tractable system. Although diverse methods to generate brain organoids have been developed, a major challenge has been the production of organoids with reproducible cell type heterogeneity and macroscopic morphology. Here, we have directly addressed this problem by establishing a robust production pipeline to generate morphologically consistent hCOs and achieve a success rate of >80%. These hCOs include both a radial glial stem cell compartment and electrophysiologically competent mature neurons. Moreover, we show using immunofluorescence microscopy and single-cell profiling that individual organoids display reproducible cell type compositions that are conserved upon extended culture. We expect that application of this method will provide new insights into brain development and disease processes. |
| Databáze: | OpenAIRE |
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