Involvement of glycosaminoglycans in the attachment of pneumococci to nasopharyngeal epithelial cells

Autor: J.H.A.J. Curfs, Theo Hafmans, Elisabeth A. M. Sanders, Paul E. Verweij, E.L.G.M. Tonnaer, Toine H. Van Kuppevelt
Rok vydání: 2006
Předmět:
Tissue engineering and reconstructive surgery [UMCN 4.3]
Immunology
Biology
medicine.disease_cause
Microbiology
Bacterial Adhesion
Dermatan sulfate
Cell Line
Glycosaminoglycan
Invasive mycoses and compromised host [N4i 2]
chemistry.chemical_compound
Nasopharynx
Streptococcus pneumoniae
medicine
Perception and Action [DCN 1]
Animals
Neurosensory disorders [UMCN 3.3]
Heart
lung and circulation [UMCN 2.1]
Pathogen
Glycosaminoglycans
Renal disorder [IGMD 9]
Epithelial Cells
Heparan sulfate
Heparin
Tissue engineering and pathology [NCMLS 3]
Epithelium
Rats
Infectious Diseases
medicine.anatomical_structure
Otitis
chemistry
Rats
Inbred Lew
Evaluation of complex medical interventions [NCEBP 2]
Female
Heparitin Sulfate
Microbial pathogenesis and host defense [UMCN 4.1]
medicine.symptom
Functional Neurogenomics [DCN 2]
Infection and autoimmunity [NCMLS 1]
medicine.drug
Immunity
infection and tissue repair [NCMLS 1]
Zdroj: Microbes and Infection, 8, 2, pp. 316-22
Microbes and Infection, 8, 316-22
ISSN: 1286-4579
Popis: Contains fulltext : 51290.pdf (Publisher’s version ) (Closed access) Streptococcus pneumoniae is a major bacterial pathogen involved in the development of otitis media. The pathogenic mechanisms of this middle ear disease, including the bacterial adherence mechanisms to the mucosal epithelial cells of the host, are poorly understood. In this study, the role of glycosaminoglycans in the adhesion of pneumococci to mucosal epithelial cells is examined. Both nasopharyngeal epithelium from rats and an oral epithelial cell line were used for pneumococcal adherence experiments. Preincubation of pneumococci with heparin, heparan sulfate (HS) and to a lesser extent, chondroitin 4-sulfate (C-4S), was found to inhibit attachment of S. pneumoniae to oral epithelial cells, while dermatan sulfate and hyaluronate did not interfere with pneumococcal binding. Enzymatic removal of HS moieties by heparinase III from nasopharyngeal epithelial cells abolished the attachment of pneumococci to nasopharyngeal epithelium. This study demonstrates that heparin, HS and C-4S are involved in pneumococcal binding to mucosal epithelial cells. This knowledge may contribute to the development of a new prophylactic strategy for otitis media.
Databáze: OpenAIRE
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