Mechanical Force Induces Phosphorylation-Mediated Signaling that Underlies Tissue Response and Robustness in Xenopus Embryos
| Autor: | Pierre M. Jean Beltran, Ileana M. Cristea, Naoto Ueno, Joel D. Federspiel, Todd M. Greco, Yutaka Hashimoto, Noriyuki Kinoshita |
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| Rok vydání: | 2018 |
| Předmět: |
Histology
Embryo Nonmammalian Proteome Xenopus Centrifugation Article Pathology and Forensic Medicine Focal adhesion 03 medical and health sciences Xenopus laevis 0302 clinical medicine Animals Kinase activity Phosphorylation Protein kinase C Protein Kinase C 030304 developmental biology Mechanical Phenomena 0303 health sciences biology Kinase Chemistry Cell Biology biology.organism_classification Cell biology Crosstalk (biology) Focal Adhesion Protein-Tyrosine Kinases Signal transduction Protein Processing Post-Translational 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Cell systems. 8(3) |
| ISSN: | 2405-4720 |
| Popis: | Mechanical forces are essential drivers of numerous biological processes, notably during development. Although it is well recognized that cells sense and adapt to mechanical forces, the signal transduction pathways that underlie mechanosensing have remained elusive. Here, we investigate the impact of mechanical centrifugation force on phosphorylation-mediated signaling in Xenopus embryos. By monitoring temporal phosphoproteome and proteome alterations in response to force, we discover and validate elevated phosphorylation on focal adhesion and tight junction components, leading to several mechanistic insights into mechanosensing and tissue restoration. First, we determine changes in kinase activity profiles during mechanoresponse, identifying the activation of basophilic kinases. Pathway interrogation using kinase inhibitor treatment uncovers a crosstalk between the focal adhesion kinase (FAK) and protein kinase C (PKC) in mechanoresponse. Second, we find LIM domain 7 protein (Lmo7) as upregulated upon centrifugation, contributing to mechanoresponse. Third, we discover that mechanical compression force induces a mesenchymal-to-epithelial transition (MET)-like phenotype. |
| Databáze: | OpenAIRE |
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