Sensitive enumeration of Listeria monocytogenes and other Listeria species in various naturally contaminated matrices using a membrane filtration method

Autor:	Bertrand Lombard, Camille Doux, Léna Barre, Emilie Brasseur, Nathalie Gnanou Besse
Rok vydání:	2015
Předmět:	Listeria digestive oral and skin physiology Colony Count Microbial Juglans Biology Contamination medicine.disease_cause Listeria monocytogenes Sensitivity and Specificity Microbiology Membrane technology law.invention Listeria species law medicine Enumeration Food science Filtration Food Science
Zdroj:	Food Microbiology. 48:171-177
ISSN:	0740-0020
DOI:	10.1016/j.fm.2014.10.012
Popis:	For the enumeration of Listeria monocytogenes ( L. monocytogenes ) in food, a sensitive enumeration method has been recently developed. This method is based on a membrane filtration of the food suspension followed by transfer of the filter on a selective medium to enumerate L. monocytogenes . An evaluation of this method was performed with several categories of foods naturally contaminated with L. monocytogenes . The results obtained with this technique were compared with those obtained from the modified reference EN ISO 11290-2 method for the enumeration of L. monocytogenes in food, and are found to provide more precise results. In most cases, the filtration method enabled to examine a greater quantity of food thus greatly improving the sensitivity of the enumeration. However, it was hardly applicable to some food categories because of filtration problems and background microbiota interference.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d809e1b7164f1c305c4689796bcce9ae https://doi.org/10.1016/j.fm.2014.10.012 Zobrazit plný text záznamu Full Text from ScienceDirect