Isozyme variation in Verticillium dahliae isolates from Crete
| Autor: | G. A. Fragkiadakis, E. K. Ligoxigakis, D. J. Vakalounakis, A. G. Manganaris, K. K. Thanassoulopoulos |
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| Rok vydání: | 2002 |
| Předmět: |
Gel electrophoresis
Polymorphism Genetic biology Greece Acid phosphatase General Medicine Fungi imperfecti Verticillium biology.organism_classification Microbiology Isozyme Esterase Molecular biology RAPD Bacterial Typing Techniques Random Amplified Polymorphic DNA Technique Isoenzymes Genetic variation biology.protein Verticillium dahliae Medicago sativa |
| Zdroj: | Folia microbiologica. 47(2) |
| ISSN: | 0015-5632 |
| Popis: | Fifteen isolates of Verticillium dahliae (eight of race 1, seven of race 2; most from the island of Crete, Greece) were examined for isozyme and molecular variation. Among the isozyme banding patterns (zymograms) of six enzymes that were "activity-stained" after electrophoresis in 9% polyacrylamide gels, differences were observed in diaphorase, alpha-esterase, peroxidase and superoxide dismutase; 2, 2, 3 and 5 different types of zymograms were recorded, respectively. The zymograms could not be correlated with either race 1 or 2. However, all six isolates originating from the Oropedio (plateau) area of Lasithi (Crete) showed an esterase zymogram clearly distinguishable from the other isolates. No differences were observed when staining for acid phosphatase or aspartate aminotransferase ('glutamic-oxaloacetic transaminase'). Furthermore, electrophoresis of random-amplified polymorphic DNA (RAPD) in 2% agarose gels showed that three race-2 isolates from Oropedio of Lasithi could also be distinguished by the RAPD pattern generated with primer OPA-1. The variation observed possibly represents adaptation of V. dahliae to the Oropedio environment. |
| Databáze: | OpenAIRE |
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