Epidemiological survey and screening strategy for dengue virus in blood donors from Yunnan Province
| Autor: | Qian Zhang, Rong Weng, John R. Hess, Ling Li, Yin Yundi, Ying Li, Yinghan Yu, Hua Shi, Zhong Liu, Shaofang Lu, Jing Dong, Ji Wu, Peng Fang, Haixia Xu, Rui He |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Adult
Male China medicine.medical_specialty Blood transfusion medicine.medical_treatment viruses Blood Donors Viral Nonstructural Proteins 030204 cardiovascular system & hematology Dengue virus Antibodies Viral medicine.disease_cause Dengue fever Serology lcsh:Infectious and parasitic diseases Dengue Screening strategy Young Adult 03 medical and health sciences 0302 clinical medicine Medical microbiology Epidemiology medicine Prevalence Humans Mass Screening Transfusion transmitted infection lcsh:RC109-216 030212 general & internal medicine business.industry virus diseases Dengue Virus Middle Aged biochemical phenomena metabolism and nutrition medicine.disease Virology Infectious Diseases Parasitology RNA Viral Female business Transfusion-transmitted infection Donor Research Article |
| Zdroj: | BMC Infectious Diseases, Vol 21, Iss 1, Pp 1-7 (2021) BMC Infectious Diseases |
| ISSN: | 1471-2334 |
| Popis: | Background Dengue virus (DENV) infection is increasingly common in southern China and can be transmitted through blood transfusion but is not currently part of donor screening throughout the region. We assessed DENV prevalence among donors at the Xishuangbanna Blood Center, Yunnan, to support development of DENV screening strategies. Methods Blood samples were collected randomly between June 2019 and August 2019. These were screened for anti-DENV IgG and IgM using enzyme-linked immunosorbent assay (ELISA). Then, all reactive samples and some randomly-chosen non-reactive samples were used to detect DENV RNAs using real-time polymerase-chain-reaction (RT-PCR) assays. After RT-PCR, samples were further tested for soluble nonstructural protein 1 (NS1) using the colloidal gold method. Donors demographics were also collected and assessed. Results Over the study period, 2254 donor samples were collected and tested for anti-DENV IgG and IgM by ELISA. This revealed 598 anti-DENV IgG and/or IgM reactive samples, a serological prevalence of 26.53%. Of these, 26 were RT-PCR positive and/or NS1 positive. Significant differences in DENV prevalence were noted by occupation (P = 0.001), education (P ), and ethnicity (P = 0.026). Conclusion The prevalence of DENV in Xishuangbanna Blood Center was higher than most other blood centers that have implemented DENV donor screening. Our study provides first-hand data about the prevalence of DENV and allows the development of a screening strategy for clinical use. |
| Databáze: | OpenAIRE |
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