Delayed reception of live blowfly (Calliphora vicina and Chrysomya rufifacies) larval samples: implications for minimum postmortem interval estimates
| Autor: | James F. Wallman, Stephanie D. Jones, Melanie S. Archer |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
sampling
Entomology Calliphora vicina forensic entomology best practice 030231 tropical medicine Zoology Biology Biochemistry Genetics and Molecular Biology (miscellaneous) Pathology and Forensic Medicine Analytical Chemistry forensic evidence larval development 03 medical and health sciences 0302 clinical medicine 030216 legal & forensic medicine Forensic entomology lcsh:K5000-5582 Physical and Theoretical Chemistry Larva lcsh:Public aspects of medicine lcsh:RA1-1270 biology.organism_classification Forensic science Psychiatry and Mental health Anthropology lcsh:Criminal law and procedure Original Article Chrysomya rufifacies |
| Zdroj: | Forensic sciences research Forensic Sciences Research, Vol 3, Iss 1, Pp 27-39 (2018) |
| ISSN: | 2471-1411 2096-1790 |
| Popis: | Forensic entomology evidence collected by police and mortuary staff may be delayed in getting to the entomologist. Live samples may continue developing and alter minimum postmortem interval (PMImin) estimates. This study investigated development of simulated evidential samples of Calliphora vicina Robineau-Desvoidy and Chrysomya rufifacies (Macquart) (Diptera: Calliphoridae) maggots. Maggots of each species were studied in three developmental classes: “small” (late second/early third instar), “mid” (mid third instar) and “large” (late third instar). Seven replicates of 11 maggots in each class were assigned without food to four treatments: (1) 24 h at 20 °C; (2) 24 h at 4 °C; (3) 48 h at 20 °C and (4) 48 h at 4 °C. There was a significant difference in absolute length change across treatments, reflecting size for C. vicina, and interaction between size, time and temperature for Ch. rufifacies. Calliphora vicina maggots showed minimal mortality, and most second instar larvae moulted by the experiment's end. Chrysomya rufifacies showed heavy mortality and minimal moulting from the second to third instar. Only “large” Ch. rufifacies maggots kept at room temperature for 48 h pupariated. Since these results confirm that development can continue in live unfed maggot samples after their collection, it is not advisable to delay their preservation. |
| Databáze: | OpenAIRE |
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