Fast Afucosylation Profiling of Glycoengineered Antibody Subunits by Middle-Up Mass Spectrometry
| Autor: | Elsa Wagner-Rousset, Yannis-Nicolas François, Stéphane Chenu, Olivier Colas, Jonathan Sjögren, Arnaud Delobel, Sarah Cianférani, Yury O. Tsybin, Davy Guillarme, Alain Beck |
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| Rok vydání: | 2021 |
| Předmět: |
Kifunensine
medicine.drug_class Monoclonal antibody Mass spectrometry 01 natural sciences EndoS2 03 medical and health sciences chemistry.chemical_compound Low fucose Obinutuzumab medicine 030304 developmental biology Antibody-dependent cell-mediated cytotoxicity ddc:615 0303 health sciences biology Chinese hamster ovary cell IdeS 010401 analytical chemistry Benralizumab 0104 chemical sciences chemistry Biochemistry biology.protein EndoS Antibody High mannose ADCC |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071612408 Mass Spectrometry of Glycoproteins. Methods in Molecular Biology |
| DOI: | 10.1007/978-1-0716-1241-5_5 |
| Popis: | Middle-up LC-MS antibody characterization workflows using reduction or IdeS digestion for a focused assessment of N-glycan profiling of three representative glycoengineered monoclonal antibodies (mAbs), namely, obinutuzumab (GlycomAb technology, Glycart/Roche), benralizumab (Potelligent Technology, BioWa, Kyowa Kirin) and mAb B (kifunensine) and compared to mAb A, produced in a common CHO cell line. In addition, EndoS or EndoS2 enzyme are used for quantitative determination of Fc-glycan core afucosylation and high mannose for these antibodies, as requested by health authorities for Fc-competent therapeutics mAbs critical quality attributes (CQAs). |
| Databáze: | OpenAIRE |
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