Dominance of Giardia assemblage B in León, Nicaragua
| Autor: | Byron Leiva, Staffan G. Svärd, Aleyda Tellez, Jan Andersson, Johan Ankarklev, Marianne Lebbad |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Adult
Giardiasis Male Adolescent Veterinary (miscellaneous) Molecular Sequence Data Protozoan Proteins Sequence Homology Nicaragua Biology Giardia intestinalis Dogs Glutamate Dehydrogenase Animals Humans Dog Diseases Child Phylogeny Genetics Molecular Epidemiology Polymorphism Genetic Ecology Giardia Infant Sequence Analysis DNA DNA Protozoan Middle Aged biology.organism_classification DNA Fingerprinting Infectious Diseases Insect Science Child Preschool Parasitology Female Polymorphism Restriction Fragment Length |
| Zdroj: | Acta tropica. 106(1) |
| ISSN: | 0001-706X |
| Popis: | Giardiasis is a major problem in León, Nicaragua, yet despite this no data are available regarding the prevalence of different Giardia genotypes in this area. To address this question, a molecular analysis of Giardia isolates from humans and dogs living in the same area in León, Nicaragua was performed. Giardia isolates from 119 Nicaraguan patients and 8 dogs were successfully genotyped using single and/or nested beta-giardin PCR with subsequent restriction length fragment polymorphism (RFLP) analysis. The analyses of human samples yielded 94 (79%) assemblage B isolates and 25 (21%) assemblage A isolates. Only the non-human-associated assemblages C and D were found in the dog samples. Sixteen isolates with assemblage A pattern, 26 isolates with assemblage B pattern and all dog isolates were further characterized by sequencing the nested beta-giardin PCR product and by molecular analyses of the glutamate dehydrogenase (gdh) gene. Within the study area the assemblage A isolates were highly genetically homogenous, showing only sub-genotypes A2 (n=3) or A3 (n=13) at the beta-giardin locus and AII only at the gdh locus while assemblage B showed a high genetic polymorphism at both loci. Seven different sub-genotypes were identified within 13 of the sequenced assemblage B beta-giardin isolates. The remaining 13 sequenced assemblage B-isolates appeared to contain several different variants of the beta-giardin gene since the chromatograms displayed one to seven double peaks. The gdh sequences showed an even higher polymorphism since only 2 of 26 assemblage B isolates were without double peaks. Two mixed infections between assemblage A and B were found when the gdh gene was analyzed. Polymorphisms were also observed in the dog-associated assemblages C and D, but to a lesser extent than in assemblage B. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect