Hypothyroidism induces uterine hyperplasia and inflammation related to sex hormone receptors expression in virgin rabbits

Autor: Dafne Zepeda-Pérez, Ignacio Camacho-Arroyo, Marlenne Castillo-Romano, Ana Gabriela Piña-Medina, Maribel Méndez-Tepepa, Estela Cuevas-Romero, Marlen Espindola-Lozano, Aylin Del Moral-Morales, Julia Rodríguez-Castelán
Rok vydání: 2019
Předmět:
Vascular Endothelial Growth Factor A
0301 basic medicine
Perilipin-1
endocrine system
medicine.medical_specialty
Uterine hyperplasia
Uterus
Gene Expression
Estrogen receptor
Endometrium
030226 pharmacology & pharmacy
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
0302 clinical medicine
Hypothyroidism
Internal medicine
medicine
Animals
General Pharmacology
Toxicology and Pharmaceutics
Gonadal Steroid Hormones
Receptor
Progesterone
Inflammation
Hyperplasia
Receptors
Thyroid Hormone
business.industry
Myometrium
General Medicine
Sex hormone receptor
Lipids
030104 developmental biology
medicine.anatomical_structure
Endocrinology
Receptors
Estrogen
Female
Rabbits
medicine.symptom
Receptors
Progesterone
business
hormones
hormone substitutes
and hormone antagonists
Hormone
Zdroj: Life Sciences. 230:111-120
ISSN: 0024-3205
DOI: 10.1016/j.lfs.2019.05.063
Popis: Aims In women, uterine alterations have been associated with sex steroid hormones. Sex hormones regulate the expression of thyroid hormone receptors (TRs) in the uterus, but an inverse link is unknown. We analyzed the impact of hypothyroidism on histological characteristics, vascular endothelial growth factor (VEGF-A), progesterone receptors (PR), estrogen receptors (ER), thyroid hormone receptors (TRs), perilipin (PLIN-A), and lipid content in the uterus of virgin rabbits. Main methods Twelve Chinchilla-breed adult female rabbits were grouped into control (n = 6) and hypothyroid (n = 6; 0.02% of methimazole for 30 days). The thickness of endometrium and myometrium, number of uterine glands, and infiltration of immune cells were analyzed. The expression of VEGF-A, PR, ERα, and PLIN-A was determined by RT-PCR and western blot. The uterine content of triglycerides (TAG), total cholesterol (TC), and malondialdehyde (MDA) was quantified. Key findings Hypothyroidism promoted uterine hyperplasia and a high infiltration of immune cells into the endometrium, including macrophages CD163+. It also increased the expression of VEGF-A, TRA, and ERα-66 but reduced that of PR and ERα-46. The uterine content of PLIN-A, TAG, and TC was reduced, but that of MDA was augmented in hypothyroid rabbits. Significance Our results suggest that uterine hyperplasia and inflammation promoted by hypothyroidism should be related to changes in the VEGF-A, PR, ER, and TRs expression, as well as to modifications in the PLIN-A expression, lipid content, and oxidative status. These results suggest that hypothyroidism should affect the fertility of females.
Databáze: OpenAIRE
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