Effect of engineered nanoparticles on vasomotor responses in rat intrapulmonary artery
| Autor: | Etienne Roux, Patrick Brochard, Mireille Canal-Raffin, Arnaud Courtois, Thomas Ducret, Francelyne Marano, Christelle Guibert, Yannick Ladeiro, Françoise Rogerieux, Ghislaine Lacroix, Roger Marthan, Pascal Andujar, Bernard Muller, I. Baudrimont |
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| Přispěvatelé: | USC 1366 Oenologie, Institut National de la Recherche Agronomique (INRA), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Génomique Fonctionnelle des Tumeurs Solides (U1162), Université Paris 13 (UP13)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] (CRCTB), Université Bordeaux Segalen - Bordeaux 2-CHU Bordeaux [Bordeaux]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de l'Environnement Industriel et des Risques (INERIS), Physiopathologie de la réactivite bronchique et vasculaire, Université Bordeaux Segalen - Bordeaux 2-Institut National de la Santé et de la Recherche Médicale (INSERM), Physiopathologie de la réactivité bronchique et vasculaire, Laboratoire Santé Travail Environnement, Université Bordeaux Segalen - Bordeaux 2-IFR99, CHU Bordeaux [Bordeaux], Université Paris Diderot - Paris 7 (UPD7), Université de Bordeaux Ségalen [Bordeaux 2], Service de pneumologie et pathologie professionnelle, CHI Créteil, Laboratoire de Cytophysiologie et Toxicologie Cellulaire, Laboratoire de physiopathologie de la nutrition (LPN), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Guellaen, Georges, Œnologie, Institut National de la Recherche Agronomique (INRA)-Université Victor Segalen - Bordeaux 2, Unité de Recherche Oenologie [Villenave d'Ornon], Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV) |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Male
Contraction (grammar) Vasodilator Agents [SDV]Life Sciences [q-bio] 02 engineering and technology MESH: Rats Sprague-Dawley Toxicology Endoplasmic Reticulum [SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract MESH: Dose-Response Relationship Drug Rats Sprague-Dawley chemistry.chemical_compound Vasoconstrictor Agents MESH: Animals MESH: Isometric Contraction ComputingMilieux_MISCELLANEOUS Titanium 0303 health sciences Inhalation Exposure Vasomotor Voltage-dependent calcium channel Biological activity 021001 nanoscience & nanotechnology MESH: Titanium MESH: Calcium Channels MESH: Inhalation Exposure 0210 nano-technology Acetylcholine medicine.drug medicine.medical_specialty Thapsigargin MESH: Rats MESH: Pulmonary Artery chemistry.chemical_element MESH: Carbon Calcium Pulmonary Artery 03 medical and health sciences MESH: Vasoconstrictor Agents In vivo MESH: Endoplasmic Reticulum Internal medicine Isometric Contraction medicine [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology Animals [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Rats Wistar 030304 developmental biology Pharmacology Dose-Response Relationship Drug MESH: Rats Wistar Carbon MESH: Male Rats MESH: Vasodilator Agents Endocrinology chemistry Nanoparticles Calcium Channels MESH: Nanoparticles [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
| Zdroj: | Toxicology and Applied Pharmacology Toxicology and Applied Pharmacology, Elsevier, 2010, 245 (2), pp.203-210. ⟨10.1016/j.taap.2010.03.002⟩ Toxicology and Applied Pharmacology, 2010, 245 (2), pp.203-10. ⟨10.1016/j.taap.2010.03.002⟩ Toxicology and Applied Pharmacology, Elsevier, 2010, 245 (2), pp.203-10. ⟨10.1016/j.taap.2010.03.002⟩ |
| ISSN: | 0041-008X 1096-0333 |
| DOI: | 10.1016/j.taap.2010.03.002⟩ |
| Popis: | International audience; Pulmonary circulation could be one of the primary vascular targets of finest particles that can deeply penetrate into the lungs after inhalation. We investigated the effects of engineered nanoparticles on vasomotor responses of small intrapulmonary arteries using isometric tension measurements. Acute in vitro exposure to carbon nanoparticles (CNP) decreased, and in some case abolished, the vasomotor responses induced by several vasoactive agents, whereas acute exposure to titanium dioxide nanoparticles (TiO(2)NP) did not. This could be attributed to a decrease in the activity of those vasoactive agents (including PGF(2)(alpha), serotonin, endothelin-1 and acetylcholine), as suggested when they were exposed to CNP before being applied to arteries. Also, CNP decreased the contraction induced by 30 mM KCl, without decreasing its activity. After endoplasmic reticulum calcium stores depletion (by caffeine and thapsigargin), CaCl(2) addition induced a contraction, dependent on Store-Operated Calcium Channels that was not modified by acute CNP exposure. Further addition of 30 mM KCl elicited a contraction, originating from activation of Voltage-Operated Calcium Channels that was diminished by CNP. Contractile responses to PGF(2)(alpha) or KCl, and relaxation to acetylcholine were modified neither in pulmonary arteries exposed in vitro for prolonged time to CNP or TiO(2)NP, nor in those removed from rats intratracheally instilled with CNP or TiO(2)NP. In conclusion, prolonged in vitro or in vivo exposure to CNP or TiO(2)NP does not affect vasomotor responses of pulmonary arteries. However, acute exposure to CNP decreases contraction mediated by activation of Voltage-Operated, but not Store-Operated, Calcium Channels. Moreover, interaction of some vasoactive agents with CNP decreases their biological activity that might lead to misinterpretation of experimental data. |
| Databáze: | OpenAIRE |
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