Oral Squamous Cell Carcinoma Contributes to Differentiation of Monocyte-Derived Tumor-Associated Macrophages via PAI-1 and IL-8 Production
| Autor: | Noriko Kakizoe-Ishiguro, Masafumi Moriyama, Kazuki Kai, Taichi Hattori, Seiji Nakamura, Shintaro Kawano, Yuka Miyahara, Akira Chinju, A. S.M.Rafiul Haque, Keigo Kubota, Chen Hu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
QH301-705.5
CD14 medicine.medical_treatment Tumor-associated macrophage Catalysis Article Flow cytometry Inorganic Chemistry tumor-associated macrophage Immune system plasminogen activator inhibitor–1 Cell Line Tumor Plasminogen Activator Inhibitor 1 Tumor-Associated Macrophages medicine Tumor Microenvironment Humans Interleukin 8 Physical and Theoretical Chemistry Biology (General) Molecular Biology QD1-999 Spectroscopy medicine.diagnostic_test integumentary system Chemistry Squamous Cell Carcinoma of Head and Neck Macrophages Organic Chemistry interleukin-8 Cell Differentiation General Medicine Immunohistochemistry Computer Science Applications oral squamous cell carcinoma stomatognathic diseases Cytokine CD206 Cell culture Head and Neck Neoplasms Cancer cell Cancer research Carcinoma Squamous Cell Leukocytes Mononuclear |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 17 International Journal of Molecular Sciences, Vol 22, Iss 9475, p 9475 (2021) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms22179475 |
| Popis: | Tumor-associated macrophages (TAMs) promote cancer cell proliferation and metastasis, as well as anti-tumor immune suppression. Recent studies have shown that tumors enhance the recruitment and differentiation of TAMs, but the detailed mechanisms have not been clarified. We thus examined the influence of cancer cells on the differentiation of monocytes to TAM subsets, including CD163+, CD204+, and CD206+ cells, in oral squamous cell carcinoma (OSCC) using immunohistochemistry, flow cytometry, and a cytokine array. Furthermore, we investigated the effect of OSCC cells (HSC-2, SQUU-A, and SQUU-B cells) on the differentiation of purified CD14+ cells to TAM subsets. The localization patterns of CD163+, CD204+, and CD206+ in OSCC sections were quite different. The expression of CD206 on CD14+ cells was significantly increased after the co-culture with OSCC cell lines, while the expressions of CD163 and CD204 on CD14+ cells showed no change. High concentrations of plasminogen activator inhibitor-1 (PAI-1) and interleukin-8 (IL-8) were detected in the conditioned medium of OSCC cell lines. PAI-1 and IL-8 stimulated CD14+ cells to express CD206. Moreover, there were positive correlations among the numbers of CD206+, PAI-1+, and IL-8+ cells in OSCC sections. These results suggest that PAI-1 and IL-8 produced by OSCC contribute to the differentiation of monocytes to CD206+ TAMs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|