Cloning and sequence analysis of the gene encoding an NADP-dependent alcohol dehydrogenase in Mycobacterium bovis BCG
| Autor: | Martine Stélandre, Yves Bosseloir, Jacqueline De Bruyn, Pierrette Maes, Jean Content |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
DNA
Bacterial Sequence analysis Molecular Sequence Data Restriction Mapping medicine.disease_cause Homology (biology) Escherichia coli Genetics medicine Amino Acid Sequence Cloning Molecular Gene Peptide sequence Alcohol dehydrogenase Electrophoresis Agar Gel Mycobacterium bovis Base Sequence Sequence Homology Amino Acid biology Alcohol Dehydrogenase Nucleic acid sequence General Medicine biology.organism_classification Molecular biology Blotting Southern Biochemistry Genes Bacterial biology.protein Electrophoresis Polyacrylamide Gel NADP hormones hormone substitutes and hormone antagonists |
| Zdroj: | Gene. 121:79-86 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/0378-1119(92)90164-k |
| Popis: | The nucleotide sequence of a 1619-bp fragment of Mycobacterium bovis BCG containing the gene that encodes an alcohol dehydrogenase (ADH) has been determined. The M(r) calculated from the deduced amino acid (aa) sequence, as well as the N terminus, are in good accordance with those determined for the ADH purified from M. bovis BCG extracts. The M. bovis BCG cloned adh gene was expressed in Escherichia coli by its own promoter and the synthesized product shows ADH activity in the butane-1-ol-NADP system. Based on comparison of the aa sequence, this enzyme belongs to the zinc-containing, long-chain alcohol/polyol dehydrogenase family, which has been primarily described in eukaryotes. Of the 22 strictly conserved residues in this group, 19 are also conserved in M. bovis BCG ADH (BCGADH). |
| Databáze: | OpenAIRE |
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